6451-73-6
6451-73-6 結(jié)構(gòu)式
基本信息
斯氏紫堇堿
(S)-金黃紫堇堿
Scoreline
l-skoulerine
l-scoulerine
SCOULERIN(SH)
Alkaloid HF-1
SCOULERIN(RG)
(-)-scoulerine
2,9-DIOXY-3,10-DIMETHOXYBERBINE
3,10-Dimethoxy-13aα-berbine-2,9-diol
物理化學(xué)性質(zhì)
常見問題列表
Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC
50
s ranging from 2.7?μM to 6.5?μM.
Scoulerine (2.5-20?μM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells.
Scoulerine (2.5-20?μM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis.
Scoulerine induces G2 or M cell cycle arrest.
Scoulerine (2.5-5?μM; 24?hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells.
Scoulerine (2.5-5?μM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner.
Scoulerine (5-10 μM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells
Cell Proliferation Assay
| Cell Line: | Jurkat and MOLT-4 cells |
| Concentration: | 2.5, 5, 10, 15 and 20?μM |
| Incubation Time: | 24?hours |
| Result: | Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner. |
Apoptosis Analysis
| Cell Line: | MOLT-4 and Jurkat cells |
| Concentration: | 2.5, 5, 10, 15 and 20?μM |
| Incubation Time: | 24 hours |
| Result: | Induced MOLT-4 and Jurkat cells apoptosis. |
Cell Cycle Analysis
| Cell Line: | Jurkat and MOLT-4 leukemic cells |
| Concentration: | 2.5-20 μM |
| Incubation Time: | 16 hours |
| Result: | Induced cell cycle arrest at the G2/M transition. |
Western Blot Analysis
| Cell Line: | MOLT-4 cells |
| Concentration: | 2.5, 5?μM |
| Incubation Time: | 24?hours |
| Result: | Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells. |