674289-55-5
中文名稱
(+/-)-BLEBBISTATIN
英文名稱
(+/-)-BLEBBISTATIN
CAS
674289-55-5
分子式
C18H16N2O2
分子量
292.33
MOL 文件
674289-55-5.mol
更新日期
2024/03/17 22:58:27
674289-55-5 結(jié)構(gòu)式
基本信息
中文別名
(+/-)-BLEBBISTATIN 英文別名
(±)Bebbistatin(R)-(+)-BLEBBISTATIN
(+/-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE
1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-β]quinolin-4-one
(+/-)-1-Phenyl-1,2,3,4-tetrahydro-4-hydroxypyrrolo[2,3-β]-7-methylquinolin-4-one
4H-Pyrrolo[2,3-b]quinolin-4-one, 1,2,3,3a-tetrahydro-3a-hydroxy-6-Methyl-1-phenyl-
(3aR)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
(3aS)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-β]quinolin-4-one
(3aR)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-β]quinolin-4-one
(+/-)-1,2,3,3A-TETRAHYDRO-3A-HYDROXY-6-METHYL-1-PHENYL-4H-PYRROLO[2,3-B]QUINOLIN-4-ONE
所屬類別
生物：生化試劑盒物理化學(xué)性質(zhì)
熔點210-212°C
沸點507.3±60.0 °C(Predicted)
密度1.31±0.1 g/cm3(Predicted)
儲存條件−20°C
溶解度可溶于氯仿（少量）、二氯甲烷、DMSO、乙酸乙酯、甲醇
酸度系數(shù)(pKa)11.15±0.20(Predicted)
形態(tài)亮黃色固體。
顏色黃色
InChIInChI=1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3
InChIKeyLZAXPYOBKSJSEX-UHFFFAOYSA-N
SMILESN1C2=C(C=C(C)C=C2)C(=O)C2(O)CCN(C3=CC=CC=C3)C=12
CAS 數(shù)據(jù)庫674289-55-5
應(yīng)用領(lǐng)域
用途1
Blebbistatin blocked myosin II-dependent cell processes. It blocks cell blebbing rapidly and reversibly. Also rapidly disrupted directed cell migration and cytokinesis in vertebrate cell. It blocks
both blebbing and cytokinesis at 50-100碌M.常見問題列表
生物活性
Blebbistatin 是一種選擇性的非肌肉肌球蛋白 II (NMII) 抑制劑，可促進(jìn)角膜內(nèi)皮細(xì)胞的定向遷移并加速傷口愈合，并更好地保留細(xì)胞連接完整性和屏障功能。靶點
Non-muscle myosin II (NMII)
體外研究
The therapeutic potential of targeting NMII to enhance CEC migration is investigated using bovine corneal endothelial cells (BCECs). Blebbistatin, a direct myosin motor inhibitor, promotes migration and directional persistence in CECs through decreasing actin retrograde flow and increasing lamellipodial protrusion persistence to accelerate wound healing in vitro.
體內(nèi)研究
Blebbistatin (0.05 mL, 20 μM; intracameral injection; daily; for 6 days; New Zealand white rabbits) treatment promotes wound healing in rabbit corneal endothelial scraping model.
| Animal Model: | New Zealand white rabbits (16-20 weeks; 3-3.5 kg) |
| Dosage: | 0.05 mL; 20 μM |
| Administration: | Intracameral injection; daily; for 6 days |
| Result: | Resulted in significant improvement of corneal clarity and corneal edema resolution, implying the restoration of an intact corneal endothelial monolayer. |