7240-37-1
7240-37-1 結(jié)構(gòu)式
基本信息
7-氨基放線菌素D
7-氨基放線菌素D(7-AAD)
7-AAD [7-氨基放線菌素 D]
7-ADD
7-AMINO-AMD
7-AAD/CH85402
7-ACTINOMYCIN C
7-AMINOACTINOMYCIN
7-AMINODACTINOMYCIN
7-AMINOACTINOMYCIN D
7-AMINOACTINOMYCIN C1
ACTINOMYCIN D, 7-AMINO
物理化學(xué)性質(zhì)
安全數(shù)據(jù)
very toxic hazardous materials
常見問題列表
7-AAD選擇性親和DNA中GC區(qū)，可用于顯影染色體中的條帶區(qū)域。
7-AAD被報道具有抗菌活性，并對某些白血病細(xì)胞株有增殖抑制活性。
- 7-AAD為潛在致癌物，請避免與皮膚眼睛直接接觸，操作中戴手套，穿實驗服。
7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry.
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AAD
negative
) and apoptotic cells (7-AAD
dim
) or dead cells (7-AAD
bright
) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells.