尿苷-5'-二磷酸二鈉鹽為有機(jī)金屬化合物，可用作生化試劑，是P2Y6受體激動(dòng)劑和GPR105受體抑制劑。
尿苷-5'-二磷酸二鈉鹽可用作生化試劑，P2Y 受體激動(dòng)劑。
Uridine-5'-diphosphate disodium salt (100 μM; for 15 min) significantly induces microglial CCL2 and CCL3 mRNA expression. Uridine-5'-diphosphate disodium salt (100 μM; 3 hours) induces chemokine expression in microglia. Uridine-5'-diphosphate disodium salt (100 μM; 0.5, 1, 3, 6 12 hours) induces expression of mRNA encoding CCL2 and CCL3 within 30 min, and such expression reaches maximal levels at 1 h, returning to basal levels at 3-12 h. Uridine-5'-diphosphate disodium salt (10, 100, 1000 μM; 3 hours) induces a concentration-dependent increase in the expression of chemokines at both the mRNA and protein level. Uridine-5'-diphosphate disodium salt (100 μM; for 15 min) induces activation of NFATc1 and NFATc2 in microglia. Uridine-5'-diphosphate disodium salt is a competitive antagonist at the human P2Y 14 receptor ( pEC 50 =7.28) but not rat P2Y 14 receptor. UDP-glucose (UDPG; HY-N7032) is a potent P2Y 14 agonist.
RT-PCR