BCECF-AM Chemische Eigenschaften,Einsatz,Produktion Methoden
R-S?tze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-S?tze Betriebsanweisung:
S22:Staub nicht einatmen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Beschreibung
BCECF AM ester is a widely-
used fluorescent indicator for estimating intracellular pH. This membrane-
permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-
life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-
dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm
vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.
Chemische Eigenschaften
pale yellow to yellow powder
Verwenden
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
Allgemeine Beschreibung
Material is an isomeric mixture of lactones, acetates, and AM esters
Biologische Aktivit?t
Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.
BCECF-AM Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
