Goralatide Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
N-Acetyl-Ser-Asp-Lys-Pro inhibits the entry of pluripotent hemopoietic stem cells into S-phase of the cell cycle and protects against Ara-C lethality in mice. It is a specific substrate for the N-terminal active site of angiotensin converting enzyme, which is responsible for its degradation in vivo.
Synthese
Fmoc-Pro-OH was coupled to a 2-chlorotriphenylmethyl chloride resin, followed by sequential addition of Lys, Asp, and Ser units via cyclic deprotection (using piperidine/DMF) and coupling (using activating reagents such as TBTU/HOBt). After peptide chain elongation, the N-terminus was acetylated with acetic anhydride. Finally, the peptide was cleaved from the resin using a trifluoroacetic acid (TFA) cleavage reagent, simultaneously removing the side-chain protecting groups. High-purity Goralatide was obtained by ether precipitation and HPLC purification.
Goralatide Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte