for western blotting of cytochrome c for caspase activation
as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
in Western blot analysis of activated caspase 3
as a primary antibody in immunodetection of rat brain sections
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Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3is mapped to human chromosome 4q35.1.
Biochem/physiol Actions
Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.
