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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
2-Methyl-5-nitro-1H-imidazole-1-acetic acid ethyl ester 2-Methyl-5-nitro-1H-imidazole-1-acetic acid ethyl ester 1016-40-6 C8H11N3O4
(E,E)-3,5-Di-O-caffeoylquinic acid (E,E)-3,5-Di-O-caffeoylquinic acid 89919-62-0 C25H24O12
IpratropiuM BroMide IMpurity F (Mixture of DiastereoMers) IpratropiuM BroMide IMpurity F (Mixture of DiastereoMers) 17812-46-3 C20H29Br2NO3
Crizotinib iMpurity Crizotinib iMpurity
(2R,3S)-2-[(1S)-1-[3,5-Bis(trifluoroMethyl)phenyl]ethoxy]-3-(4-fluorophenyl)Morpholine hydrochloride (2R,3S)-2-[(1S)-1-[3,5-Bis(trifluoroMethyl)phenyl]ethoxy]-3-(4-fluorophenyl)Morpholine hydrochloride 1185503-48-3 C20H18F7NO2
SalbutaMol IMpurity K SalbutaMol IMpurity K 898542-80-8 C13H18ClNO3
TaMsulosin EP IMpurity C TaMsulosin EP IMpurity C C20H28N2O5S
Desacetyl DesMethyl LacosaMide Desacetyl DesMethyl LacosaMide 175481-39-7 C10H14N2O2
1-(4-AMinophenyl)-5,6-dihydro-3-(4-Morpholinyl)-2(1h)-pyridinone 1-(4-AMinophenyl)-5,6-dihydro-3-(4-Morpholinyl)-2(1h)-pyridinone 1267610-26-3 C15H19N3O2
Meropenem IMP A Meropenem IMP A 1053703-36-8 C17H27N3O6S
Dabigatran Impurity 8 Dabigatran Impurity 8 1408238-40-3 C34H40N6O6
Tedizolid IMpurity Tedizolid IMpurity 1256966-04-7 C37H34F2N14O11P2
Bromhexine EP Impurity E Bromhexine EP Impurity E 1660957-93-6 C15H21Br2ClN2
2-(4-(sec-butoxy)-3-cyanophenyl)-4-methylthiazole-5-carboxylic acid 2-(4-(sec-butoxy)-3-cyanophenyl)-4-methylthiazole-5-carboxylic acid 1335202-59-9 C16H16N2O3S
Ascorbic acid EP Impurity G (L-threo-Hex-2-eneric acid, 1-4-lactone) Ascorbic acid EP Impurity G (L-threo-Hex-2-eneric acid, 1-4-lactone) 66757-69-5 C6H6O7
2-(Acetylamino)-N-(phenylmethyl)-2-propenamide 2-(Acetylamino)-N-(phenylmethyl)-2-propenamide 86921-49-5 C12H14N2O2
Formoterol EP Impurity F Formoterol EP Impurity F 1795129-59-7 C37H46N4O6
Tizanidine Impurity Tizanidine Impurity
(R)-2-(1-((((R)-1-(3-((E)-2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-(2-(2-hydroxypropan-2-yl)phenyl)prop (R)-2-(1-((((R)-1-(3-((E)-2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-(2-(2-hydroxypropan-2-yl)phenyl)prop
N-(2-Amino-2-oxoethyl)-N-[(2S)-2-(3,5-dioxo-1-piperazinyl)propyl]-glycine N-(2-Amino-2-oxoethyl)-N-[(2S)-2-(3,5-dioxo-1-piperazinyl)propyl]-glycine 120418-76-0 C11H18N4O5
Canagliflozin Impurity 16 Canagliflozin Impurity 16 1807632-93-4 C15H14Br2O
Dapagliflozin Impurity 8 Dapagliflozin Impurity 8 2305949-33-9 C15H12ClIO2
Everolimus Impurity 1 Everolimus Impurity 1 745779-75-3 C52H81NO14
Peramivir Impurity 1 Peramivir Impurity 1 1988779-13-0 C18H34N2O5
Trillikamtoside Q Trillikamtoside Q 2098642-70-5 C49H72O25
Lenalidomide Impurity 13 Lenalidomide Impurity 13 874760-71-1 C13H13N3O6
Paliperidone Impurity 7 Paliperidone Impurity 7 1415488-05-9 C27H45ClN2O3
DIHYDROCYCLOSPORIN A DIHYDROCYCLOSPORIN A 59865-15-5 C62H113N11O12
N-ACETYLNEURAMIN-LACTOSE N-ACETYLNEURAMIN-LACTOSE 35890-39-2 C23H39NO19
8-cyclopentyl-5-methyl-2-((5-(piperazin-1-yl)pyridin-2-yl)amino)-6-vinylpyrido[2,3-d]pyrimidin-7(8H)-one 8-cyclopentyl-5-methyl-2-((5-(piperazin-1-yl)pyridin-2-yl)amino)-6-vinylpyrido[2,3-d]pyrimidin-7(8H)-one 2204863-06-7 C24H29N7O
Irinotecan Impurity 18 Irinotecan Impurity 18 1255644-71-3 C33H38N4O6
Erythromycin Impurity 9 Erythromycin Impurity 9 58781-38-7 C38H63NO13
Rasagiline Dipropynyl HCl Rasagiline Dipropynyl HCl 92850-02-7 C15H15N
Bortezomib Impurity Z Bortezomib Impurity Z
Bortezomib Impurity Z Bortezomib Impurity Z
Cilastatin Impurity B Cilastatin Impurity B
Brivaracetam Impurity 21 Brivaracetam Impurity 21 63095-60-3 C7H12O2
Afatinib Impurity des-EJA Afatinib Impurity des-EJA 2223677-64-1 C22H20ClFN4O3
Parecoxib Sodium EJF Parecoxib Sodium EJF 2338845-31-9 C32H24N2O4S
Deferasirox Impurity 13 Deferasirox Impurity 13
Doxofylline iMpurity D Doxofylline iMpurity D 67236-15-1 C19H28ClNO3
N-Pyrazinylcarbonyl-L-phenylalanine Methyl Ester N-Pyrazinylcarbonyl-L-phenylalanine Methyl Ester 73058-37-4 C15H15N3O3
Piperacillin impurity 8/Piperacillin Dimer impurity 3 Piperacillin impurity 8/Piperacillin Dimer impurity 3 2170771-53-4 C46H54N10O14S2
Demeclocycline Impurity E(EP) Demeclocycline Impurity E(EP) 22756-01-0 C21H20N2O7
rac-Ibuprofen amide rac-Ibuprofen amide 59512-17-3 C13H19NO
D-glycero-Heptaric acid, 2,4,6-trideoxy-2-[(1R)-1-hydroxyethyl]-6-imino-5-S-[2-[(iminomethyl)amino]ethyl]-5-thio-, 7,3-lactone, (3ξ,5ξ)- D-glycero-Heptaric acid, 2,4,6-trideoxy-2-[(1R)-1-hydroxyethyl]-6-imino-5-S-[2-[(iminomethyl)amino]ethyl]-5-thio-, 7,3-lactone, (3ξ,5ξ)- 1193105-97-3 C12H19N3O5S
1H-Imidazole-5-carboxylic acid, 1-methyl-4-[methyl[[(5-oxohexyl)amino]carbonyl]amino]- 1H-Imidazole-5-carboxylic acid, 1-methyl-4-[methyl[[(5-oxohexyl)amino]carbonyl]amino]- 84321-40-4 C13H20N4O4
1H-Imidazole-5-carboxamide, 1-methyl-4-(methylamino)-N-(5-oxohexyl)- 1H-Imidazole-5-carboxamide, 1-methyl-4-(methylamino)-N-(5-oxohexyl)- 70486-28-1 C12H20N4O2
3-chloro-N,N-dimethyl-1-phenylpropan-1-amine hydrochloride 3-chloro-N,N-dimethyl-1-phenylpropan-1-amine hydrochloride 81402-53-1 C11H17Cl2N
Montelukast sodium EP Impurity J Montelukast sodium EP Impurity J
2,3-dihydro-2-methyl-1H-indol-1-amine monomethanesulphonate       2,3-dihydro-2-methyl-1H-indol-1-amine monomethanesulphonate 85392-00-3 C10H16N2O3S
5-[ethyl(2-hydroxyethyl)amino]pentan-2-one 5-[ethyl(2-hydroxyethyl)amino]pentan-2-one 74509-79-8 C9H19NO2
Impurity reference substance Impurity reference substance
(2b,3a,5a,16b,17b)-17-Acetoxy-3-hydroxy-2-(4-morpholinyl)-16-(1-pyrrolidinyl)androstane (2b,3a,5a,16b,17b)-17-Acetoxy-3-hydroxy-2-(4-morpholinyl)-16-(1-pyrrolidinyl)androstane 119302-24-8 C29H48N2O4
Butanoic acid, 2,4-dicyano-3-oxo-, ethyl ester Butanoic acid, 2,4-dicyano-3-oxo-, ethyl ester 861612-04-6 C8H8N2O3
D-threo-Pentitol, 2,5-anhydro-1,3,4-trideoxy-2-C-(4-fluorophenyl)-4-(hydroxymethyl)-1-(1H-1,2,4-triazol-1-yl)- D-threo-Pentitol, 2,5-anhydro-1,3,4-trideoxy-2-C-(4-fluorophenyl)-4-(hydroxymethyl)-1-(1H-1,2,4-triazol-1-yl)- 1978305-26-8 C14H16FN3O2
Bromhexine Glucose Compound 1 Bromhexine Glucose Compound 1 3049873-97-1 C20H30Br2N2O5
2-CHLORO-1-(4-ISOBUTYLPHENYL)PROPAN-1-ONE 2-CHLORO-1-(4-ISOBUTYLPHENYL)PROPAN-1-ONE 80336-66-9 C13H17ClO
Omeprazole EP Impurity F Omeprazole EP Impurity F 125656-82-8 C16H13N3O2S
1-METHYL-6,7-DIHYDROXY-3,4-DIHYDROISOQUINOLINE MONOHYDRATE 1-METHYL-6,7-DIHYDROXY-3,4-DIHYDROISOQUINOLINE MONOHYDRATE 27740-96-1 C10H13NO2
penicillin X penicillin X 525-91-7 C16H18N2O5S
curculigoside B curculigoside B 143601-09-6 C21H24O11
taspine taspine 602-07-3 C20H19NO6
Batatasin  I Batatasin I 51415-00-0 C17H16O4
(-)-4-(4-Dimethylamino)-1-(4-fluorophenyl)-1-(hydroxybuty)-3-hydroxymethyl)-benzonitrile hemi D-(+)-di-p-toloyltartaric acid salt (-)-4-(4-Dimethylamino)-1-(4-fluorophenyl)-1-(hydroxybuty)-3-hydroxymethyl)-benzonitrile hemi D-(+)-di-p-toloyltartaric acid salt 128173-53-5 2C20H23FN2O2.C20H18O8
(3,4-Dichlorobenzyl)methylamine (3,4-Dichlorobenzyl)methylamine 5635-67-6 C8H9Cl2N
N-Desmethyl Dapoxetine N-Desmethyl Dapoxetine 157166-71-7 C20H22ClNO
LYSERGOL LYSERGOL 602-85-7 C16H18N2O
(1β,5β)-8-Methyl-8-azabicyclo[3.2.1]octa-6-ene-3α-ol (1β,5β)-8-Methyl-8-azabicyclo[3.2.1]octa-6-ene-3α-ol 20513-09-1 C8H13NO
N-Methyl-5-methylamino-1H-imidazole-4-carboxamide N-Methyl-5-methylamino-1H-imidazole-4-carboxamide 6736-40-9 C6H10N4O
(R)-(-)-MUSCONE (R)-(-)-MUSCONE 10403-00-6 C16H30O
3-Isobutylglutarimid 3-Isobutylglutarimid 916982-10-0 C9H15NO2
Dehydroxy Bisoprolol Dehydroxy Bisoprolol 1217245-60-7 C18H29NO3
6α-Hydroxy Norgestrel 6α-Hydroxy Norgestrel 87585-03-3 C21H28O3
FaMotidine Acid IMpurity FaMotidine Acid IMpurity 107880-74-0 C8H12N4O2S2
N-Desbutyl-N-propyl BuMetanide N-Desbutyl-N-propyl BuMetanide 28395-28-0 C16H18N2O5S
(1S,2S,3S,4R)-Methyl 3-((R)-1-aMino-2-ethylbutyl)-4-(tert-butoxycarbonylaMino)-2-hydroxycyclopentanecarboxylate (1S,2S,3S,4R)-Methyl 3-((R)-1-aMino-2-ethylbutyl)-4-(tert-butoxycarbonylaMino)-2-hydroxycyclopentanecarboxylate 316173-29-2 C18H34N2O5
Amoxicillin Related Compound D (50 mg) ((4S)-2-{[(R)-2-amino-2-(4-hydroxyphenyl)acetamido](carboxy)methyl}-5,5-dimethylthiazolidine-4-carboxylic acid, monosodium salt) Amoxicillin Related Compound D (50 mg) ((4S)-2-{[(R)-2-amino-2-(4-hydroxyphenyl)acetamido](carboxy)methyl}-5,5-dimethylthiazolidine-4-carboxylic acid, monosodium salt) 68728-47-2 C16H20N3NaO6S
Cyclophosphamide Related Compound D (25 mg) (3-[2-(2-chloroethylamino)ethylamino]propyl dihydrogen phosphate dihydrochloride) Cyclophosphamide Related Compound D (25 mg) (3-[2-(2-chloroethylamino)ethylamino]propyl dihydrogen phosphate dihydrochloride) 158401-51-5 C7H19Cl2N2O4P
3-Quinolinecarboxylic acid, 7-aMino-1-cyclopropyl-6-fluoro-1,4-dihydro-8-Methoxy-4-oxo- 3-Quinolinecarboxylic acid, 7-aMino-1-cyclopropyl-6-fluoro-1,4-dihydro-8-Methoxy-4-oxo- 172426-88-9 C14H13FN2O4
EsoMeprazole related substance H431/41 EsoMeprazole related substance H431/41 1227380-90-6 C16H15N3O4
AMpicillin EP IMpurity C AMpicillin EP IMpurity C 49841-96-5 C16H19N3O4S
9β,10α-Pregna-4,6,8(14)-triene-3,20-dione 9β,10α-Pregna-4,6,8(14)-triene-3,20-dione 23035-53-2 C21H26O2
N-(3,4-diMethoxyphenethyl)-4-(4-Methoxyphenyl)butan-2-aMine N-(3,4-diMethoxyphenethyl)-4-(4-Methoxyphenyl)butan-2-aMine 61413-44-3 C21H29NO3
Dabigatran Etexilate iMpurity H Dabigatran Etexilate iMpurity H 1702936-92-2 C19H20N4O3
RebaMipide 3-Chloro IMpurity RebaMipide 3-Chloro IMpurity 90098-05-8 C19H15ClN2O4
Solifenacin IMpurity Solifenacin IMpurity 1956436-64-8 C23H26N2O3
Methotrexate γ-Methyl Ester Methotrexate γ-Methyl Ester 67022-39-3 C21H24N8O5
RebaMipide iMpurity 1 RebaMipide iMpurity 1 100342-53-8 C18H15ClN2O2
7-Chlorobenzo[b]thiophene-3-Methanol 7-Chlorobenzo[b]thiophene-3-Methanol 142181-53-1 C9H7ClOS
N,5-bis(4-chlorophenyl)-3-iMino-3,5-dihydrophenazin-2-aMine N,5-bis(4-chlorophenyl)-3-iMino-3,5-dihydrophenazin-2-aMine 102262-55-5 C24H16Cl2N4
Milnacipran Related Impurity 3 Milnacipran Related Impurity 3 19532-98-0 C10H12N2O2
1,1′-Trimethylenedi-theobromine 1,1′-Trimethylenedi-theobromine 74857-22-0 C17H20N8O4
Salbutamol EP Impurity D Salbutamol EP Impurity D C13H21NO3
Atazanavir EP Impurity G Atazanavir EP Impurity G 1332981-16-4 C38H52N6O7
1-(4-Bromobutyl)-3,4-dihydro-7-hydroxy-2(1H)-quinolinone 1-(4-Bromobutyl)-3,4-dihydro-7-hydroxy-2(1H)-quinolinone 1424857-68-0 C13H16BrNO2
Pitavastatin Pitavastatin 154170-27-1 C32H36FNO4
Famotidine EP Impurity A Famotidine EP Impurity A 124646-10-2 C8H14N6S2
(6-methyl-2-p-tolyl-2,3-dihydro-imidazo[1,2-a]pyridin-3-yl)-oxoacetic acid (6-methyl-2-p-tolyl-2,3-dihydro-imidazo[1,2-a]pyridin-3-yl)-oxoacetic acid 927820-48-2 C17H14N2O3
Rivaroxaban Related Compound (R-4-(4-(5-(Aminomethyl)-2-Oxooxazolidin-3-yl)phenyl-Morpholin-3-One HCl) Rivaroxaban Related Compound (R-4-(4-(5-(Aminomethyl)-2-Oxooxazolidin-3-yl)phenyl-Morpholin-3-One HCl) 1429311-47-6 C14H18ClN3O4
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