CHAT膽堿乙酰轉(zhuǎn)移酶抗體
| 中文名稱 | CHAT膽堿乙酰轉(zhuǎn)移酶抗體 |
|---|---|
| 中文同義詞 | CHAT膽堿乙酰轉(zhuǎn)移酶抗體 |
| 英文名稱 | Anti-CHAT Polyclonal Antibody |
| 英文同義詞 | Anti-CHAT Polyclonal Antibody;Rabbit Anti-ChAT antibody |
| CAS號 | |
| 分子式 | |
| 分子量 | 0 |
| EINECS號 | |
| 相關(guān)類別 | |
| Mol文件 | Mol File |
| 結(jié)構(gòu)式 |
CHAT膽堿乙酰轉(zhuǎn)移酶抗體 性質(zhì)
Anti-CHAT/Choline Acetyltransferase Antibody, Rabbit Polyclonal-Cat: 100420-T10 ---Sino Biological
Immunofluorescence staining of CHAT in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CHAT polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
CHAT膽堿乙酰轉(zhuǎn)移酶抗體的純化方法:蛋白A親和純化。
Congenital myasthenic syndromes (CMS) are neuromuscular transmission disorders caused by mutations in genes encoding neuromuscular junction proteins. CMS due to choline acetyltransferase (CHAT) gene is characterized by episodic apnea. The CHAT gene encodes choline acetyltransferase, which is an enzyme responsible for the biosynthesis of the neurotransmitter acetylcholine in the brain. The choline O-acetyltransferase (CHAT) gene has been associated with various human disorders that involve cognitive impairment or deficiency. Choline acetyltransferase (CHAT) gene mutations cause a rare presynaptic congenital myasthenic syndrome due to impaired acetylcholine resynthesis.膽堿乙酰轉(zhuǎn)移酶是一種在神經(jīng)元胞體內(nèi)合成的酶。當(dāng)該轉(zhuǎn)移酶被合成以后，通過軸質(zhì)流動(dòng)方式轉(zhuǎn)移到神經(jīng)軸突末端。其功能是將乙酰輔酶A轉(zhuǎn)移到膽堿上，導(dǎo)致神經(jīng)遞質(zhì)乙酰膽堿的形成。既往研究發(fā)現(xiàn)膽堿乙酰轉(zhuǎn)移酶為MG胸腺的差異表達(dá)蛋白之一。CHAT作為非神經(jīng)元型膽堿能系統(tǒng)的關(guān)鍵蛋白，近年來受到較多關(guān)注。
CHAT膽堿乙酰轉(zhuǎn)移酶抗體的保存條件:4℃運(yùn)輸，-20℃保存，避免反復(fù)凍融。CHAT膽堿乙酰轉(zhuǎn)移酶抗體是以CHAT膽堿乙酰轉(zhuǎn)移酶為抗原的多克隆抗體，可以特異性結(jié)合CHAT膽堿乙酰轉(zhuǎn)移酶。CHAT膽堿乙酰轉(zhuǎn)移酶抗體主要用于ICC/IF,Dotblot,ELISA,IHC-P,IHC-Fr,Immunomicroscopy,WB等CHAT膽堿乙酰轉(zhuǎn)移酶檢測實(shí)驗(yàn)。