Anti-EGFR Antibody, Mouse MonoclonalMouseHumanIHC-Fr, ICC/IF, Flow CytRecombinant Human EGFR / HER1 / ErbB1 proteinThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (ab30)ab30 staining EGFR (colored green) in A431 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30 at 5μg/ml overnight at 4ºC. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

EGFR是表皮生長因子受體（HER）家族成員之一。該家族包括HER1（erbB1，EGFR）、HER2（erbB2，NEU）、HER3（erbB3）及HER4（erbB4）。HER家族在細胞生理過程中發(fā)揮重要的調(diào)節(jié)作用。 EGFR（Epidermal Growth Factor Receptor）是上皮生長因子（EGF）細胞增殖和信號傳導的受體。EGFR屬于ErbB受體家族的一種，該家族包括EGFR (ErbB-1),HER2/c-neu(ErbB-2),Her 3(ErbB-3) 和Her 4(ErbB-4)。EGFR也被稱作HER1、ErbB1，突變或過表達一般會引發(fā)腫瘤。EGFR是一種糖蛋白，屬于酪氨酸激酶型受體，細胞膜貫通，分子量170KDa。EGFR位于細胞膜表面，靠與配體結(jié)合來激活，包括EGF和TGFα（transforming growth factor α）。激活后，EGFR由單體轉(zhuǎn)化為二聚體，盡管也有證據(jù)表明，激活前也存在二聚體。小鼠抗EGFR單克隆抗體是一類可以特異性結(jié)合EGFR的單克隆抗體，主要用于檢測EGFR的Western Blot、IHC-P、IF、ELISA、Co-IP等多種免疫學實驗。雙抗體夾心法測定標本中EGFR水平。用純化的Anti-ACTA2抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入EGFR，再與HRP標記的EGFR抗體結(jié)合，形成抗體-抗原-酶標抗體復合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的EGFR呈正相關(guān)。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中EGFR濃度。