鹽酸石蒜堿
鹽酸石蒜堿 性質(zhì)
| 熔點(diǎn) | 206 °C |
|---|---|
| 儲(chǔ)存條件 | Keep in dark place,Inert atmosphere,2-8°C |
| 溶解度 | 溶于水(15mg/毫升) |
| 形態(tài) | 粉末 |
| 顏色 | 白色至類白色 |
| 穩(wěn)定性 | 在-20°C下的水溶液可保存長(zhǎng)達(dá)1個(gè)月。 |
| InChI | 1S/C16H17NO4.ClH/c18-11-3-8-1-2-17-6-9-4-12-13(21-7-20-12)5-10(9)14(15(8)17)16(11)19;/h3-5,11,14-16,18-19H,1-2,6-7H2;1H/t11-,14-,15+,16+;/m0./s1 |
| InChIKey | VUVNTYCHKZBOMV-NVJKKXITSA-N |
| SMILES | Cl.O[C@H]1C=C2CCN3Cc4cc5OCOc5cc4[C@H]([C@@H]1O)[C@@H]23 |
Lycorine hydrochloride 是石蒜的主要活性成分，也是黑色素瘤血管生成抑制劑，具有抗腫瘤活性。Lycorine hydrochloride 能有效抑制 Hey1B 細(xì)胞的有絲分裂增殖 (IC50 為 1.2 μM)。
IC50: 1.2 μM (Hey1B cells )
Lycorine hydrochloride (0-25 μM; 48 hours; C8161 cells) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected.
Lycorine hydrochloride (0-25 μM; 48 hours; C8161 cells) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion.
RT-PCR
| Cell Line: | C8161 cells |
| Concentration: | 0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM |
| Incubation Time: | 48 hours |
| Result: | Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells. |
Western Blot Analysis
| Cell Line: | C8161 cells |
| Concentration: | 0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM |
| Incubation Time: | 48 hours |
| Result: | Markedly reduced VE-cadherin protein levels in C8161 cells in a dose-dependent fashion. |
Lycorine hydrochloride (0-15 μg; subcutaneous injection; for 14 days; BALB/C nude mice) treatment has an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry.
| Animal Model: | 6- to 8-week-old BALB/C nude mice |
| Dosage: | 0 μg, 7.5 μg, 15 μg |
| Administration: | Subcutaneous injection; for 14 days |
| Result: | Showed an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry. |
化學(xué)性質(zhì)
白色結(jié)晶粉末，可溶于甲醇、乙醇、DMSO等有機(jī)溶劑，來(lái)源于石蒜鱗莖。用途
一種選擇性的PTC抑制劑。用途
肽轉(zhuǎn)移酶中心（peptidyl transferase center ，PTC）的選擇性抑制劑。