兔多克隆抗體to Bax

Rabbit

Preliminary customer data suggests that the current lot may not work in rat species, therefore it will be removed as a validated species until new testing is available.

與反應(yīng): Human 預(yù)測可用于: Rat

Synthetic peptide corresponding to Human Bax. The immunogen in within aa 1-50. Database link: Q07812

Western blot - Anti-Bax antibody (ab53154)
All lanes : Anti-Bax antibody (ab53154) at 1/500 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide
Predicted band size: 21 kDa
Observed band size: 21 kDa

BAX抗體是以人工合成的Bax氨基端(Amino-terminal)的一段多肽為抗原制備而成的抗Bax單克隆抗體，是應(yīng)用最廣的Bax抗體?？贵w類型為Mouse IgG1。Bax可以形成寡聚體，然后從細(xì)胞漿中轉(zhuǎn)移到線粒體膜上；通過和線粒體膜上的一些pore proteins相互作用，增強(qiáng)線粒體的通透性(permeability)；最后導(dǎo)致細(xì)胞色素c釋放(cytochrome c release)、caspase-9激活以及后續(xù)的其它c(diǎn)aspase激活和最終的細(xì)胞凋亡。Bax可以和Bcl-2形成多聚體，來調(diào)節(jié)線粒體膜的通透性，最終調(diào)節(jié)細(xì)胞的凋亡或存活。BAX是與BCL-2同源的水溶性相關(guān)蛋白，是BCL-2基因家族中細(xì)胞凋亡促進(jìn)基因，BAX的過度表達(dá)可拮抗BCL-2的保護(hù)效應(yīng)而使細(xì)胞趨于死亡。

Bax抗體可以檢測內(nèi)源性水平的Bax，未發(fā)現(xiàn)和其它Bcl-2家族蛋白有交叉反應(yīng)。Bax是一種促凋亡蛋白，上調(diào)Bax蛋白水平可以促進(jìn)細(xì)胞凋亡。在通過線粒體應(yīng)激誘導(dǎo)的細(xì)胞凋亡中Bax起關(guān)鍵作用。其可用于bcl-2和bax對肝癌細(xì)胞凋亡的調(diào)控及肝癌凋亡相關(guān)單克隆抗體初步研究。