公司官網(wǎng) http://www.staherb.com/ 資質(zhì):HALAL, KOSHER等認證
?：19573130018 楊經(jīng)理（微信同號）
長沙上禾生物·育亨賓提取物（Yohimbine Extract）專業(yè)生產(chǎn) 中英文簡介
一、中文簡介（Professional Production — Chinese）
工藝路線總覽：
上禾生物育亨賓提取物生產(chǎn)分兩條主線：
標準化樹皮提取物（Yohimbine 2%~20% HPLC，比例 4:1~20:1）——樹皮前處理 → 酸醇提取 → 堿沉/酸沉除雜 → 大孔吸附樹脂富集 → 噴霧干燥；
高純育亨賓堿單體 / 鹽酸育亨賓（≥98% HPLC）——標化提取物經(jīng)大孔/離子交換樹脂深度純化、硅膠/ODS柱層析、酸轉(zhuǎn)化成鹽、有機溶劑重結(jié)晶精制。
關(guān)鍵生產(chǎn)工序：
① 原料前處理（關(guān)鍵控制點）
選用喀麥隆、剛果、加蓬等西非/中非產(chǎn)區(qū)的育亨賓樹（Pausinystalia yohimbe）干燥樹皮，優(yōu)選15~20年生成年樹皮，入廠檢測育亨賓堿基線≥0.8%~1.5% DW（拒收<0.5%~0.8%），嚴控水分≤10%、霉變、重金屬及非Pausinystalia樹皮混入，附CITES/當(dāng)?shù)睾戏ú煞ノ募?/p>
干樹皮除雜 → 粉碎過20~40目篩（過細易帶入木質(zhì)素/樹脂雜質(zhì)堵柱，過粗生物堿提取不盡）；
育亨賓堿具吲哚喹啉結(jié)構(gòu)，對熱、光、強酸強堿敏感，前處理與存儲須避光、低溫、控pH。
② 酸醇提?。ˋcid-Alcohol Extraction —— 生物堿溶出核心）
育亨賓在植物中以生物堿鹽或游離弱堿存在，難溶于水，易溶于醇及酸水溶液。采用 50%~90% 食品級乙醇 + 0.05~0.1 mol/L 稀硫酸/鹽酸 調(diào)pH 3~4 的酸醇混合溶劑，料液比1:5~1:10，70~85℃ 回流或超聲輔助提取 2~3次（1~2h/次），利用H?使生物堿成鹽增溶。
合并提取液 → 板框/離心過濾 → 減壓濃縮（≤60℃、N?保護） 回收乙醇至適當(dāng)比重（1.10~1.20），防熱敏降解；也可用超臨界CO?（加夾帶劑）綠色提取但成本高。
③ 除雜——堿沉/酸沉與脫脂（Impurity Removal）
④ 純化富集——大孔吸附/離子交換樹脂（Core Purification）
⑤ 濃縮與標化干燥
⑥ 高純單體精制（Yohimbine Base ≥98% / Yohimbine HCl ≥98%）
路線A（游離堿重結(jié)晶）：標化提取物溶于熱無水乙醇/甲醇/乙酸乙酯，加0.1%~0.5% 活性炭脫色（40~60℃攪拌），熱濾 → 濾液低溫（2~5℃）緩慢析晶 → 過濾得粗結(jié)晶 → 重復(fù)乙酸乙酯/乙醇-水重結(jié)晶2~3次 → 真空干燥（≤40℃）得白色至類白色育亨賓堿（≥98% HPLC），單雜（偽/α/β型）<0.1%~0.5%。
路線B（鹽酸育亨賓成鹽）：游離堿或高純提取物溶于少量稀鹽酸-乙醇溶液，調(diào)pH 3~4 成鹽 → 濃縮 → 乙醇-水/丙酮-水體系重結(jié)晶 → 過濾得鹽酸育亨賓（Yohimbine Hydrochloride，≥98%~99% 白色結(jié)晶），水溶性顯著提升；
全程避光、控濕（生物堿鹽引濕）、充氮防氧化變色。
⑦ 質(zhì)控與包裝
檢測：HPLC測育亨賓（主峰及偽育亨賓、α/β-育亨賓、柯楠堿等有關(guān)生物堿）、手性純度評估；UV輔助；檢查干燥失重、熾灼殘渣、重金屬（Pb≤1~10ppm等）、酸根（Cl?/SO?2?）殘留、溶劑殘留（乙醇/甲醇/乙酸乙酯）、微生物；
粉末/晶體：內(nèi)雙層PE+棕色玻璃瓶/鋁箔袋（充氮/真空）；嚴格避光、2~8℃冷藏或-20℃冷凍（生物堿光敏易降解，鹽酸鹽引濕）；保質(zhì)期24個月；
年產(chǎn)能：育亨賓樹皮提取物數(shù)十噸級（2%~20%），高純育亨賓堿/鹽酸鹽因原料含量極低、分離難，按訂單百克至公斤級定制；
質(zhì)量體系：ISO 9001 / GMP / ISO 22000 / HALAL / KOSHER；可提供COA、MSDS、典型HPLC色譜圖、CITES合規(guī)文件。
二、English Introduction (Professional Production — English)
Process Route Overview:
Staherb produces two main categories of Yohimbine Extract:
Standardized Bark Extract (Yohimbine 2%~20% HPLC, Ratio 4:1~20:1) — Bark pre-treatment → Acid-alcohol extraction → Alkali/acid precipitation → Macroporous/Ion-exchange resin enrichment → Spray drying.
High-purity Yohimbine Base / Yohimbine Hydrochloride (≥98% HPLC) — Standardized extract further purified via deep macroporous/IEX resin, silica/ODS column chromatography, salification (HCl), and organic solvent recrystallization.
Key Production Steps:
① Raw Material Pre-treatment
Source: Dried bark of Pausinystalia yohimbefrom Cameroon, Congo, Gabon (West/Central Africa), prefer 15–20 yr mature bark. Test: Yohimbine baseline ≥0.8%~1.5% DW (reject<0.5%~0.8%), moisture ≤10%, mold, heavy metals, non-Pausinystaliaadmixture, with CITES/local legal harvest docs. Debris removed → milled 20–40 mesh (too fine introduces lignin/resin clogging; too coarse poor alkaloid extraction). Yohimbine (indole-quinolizidine) is light/heat/extreme acid-base sensitive; pre-treatment/storage requires light protection, low-temp, pH control.
② Acid-Alcohol Extraction (Core Alkaloid Solubilization)
Yohimbine exists as alkaloidal salts or free weak base, poorly water-soluble but soluble in alcohols/acids. Extract with 50%–90% food-grade EtOH + 0.05–0.1 mol/L H?SO?/HCl (pH 3–4), 1:5–10 w/v, 70–85℃ reflux or ultrasound-assisted 2–3× (1–2h). H? converts base to salt for solubilization. Combine → filter → vacuum conc. (≤60℃, N?) to rel. dens. 1.10–1.20. Alternately supercritical CO? (with entrainer) for green extraction at higher cost.
③ De-impurity: Alkali/Acid PPT & Degreasing
Alkalinization: Adjust conc. to pH 8–10 with NH?OH, Na?CO? to precipitate impurities (resins, tannins, pigments) or precipitate free alkaloid for crude separation → filter.
Acid re-dissolution / Water precipitation: Precipitate redissolved in dilute acid → filter remove lignin/waxes; or concentrate diluted with water to water-precipitate polysaccharides/proteins/colloids.
Optionally petroleum ether/n-hexane degreasing to remove chlorophyll/fats.
④ Purification — Macroporous / Ion-Exchange Resin (Core)
Diluted acid soln loaded onto macroporous (AB-8, D101, LX-18, LS-300) or weak acid cation-exchange resins: water wash → 10%–50% EtOH/MeOH remove flavonoids, weak alkaloids, low-polar impurities → 70%–80% EtOH (or pH 8–10 alk. alcohol) gradient elution to collect Yohimbine & accompanying alkaloids (HPLC tracked, recovery 70%–80%).
For separating Pseudoyohimbine, α/β-Yohimbine, Corynanthine, secondary column or ODS/silica gel chromatography may be used.
⑤ Concentration & Standardized Drying
Eluate conc. under reduced pressure (≤50℃) → spray-dry (inlet 150–160℃, outlet 70–80℃, with antioxidant wall material) or vacuum/freeze-dried to brownish-yellow to brown powder (2%~20% Yohimbine).
⑥ High-purity Monomer (≥98% Base / HCl)
Route A (Base Recrystallization): Standardized extract dissolved in hot anhydrous EtOH/MeOH/EA, add 0.1%–0.5% activated carbon (40–60℃ stir) → hot filter → slow recrystallization at 2–5℃ → filter crude → repeat EA/EtOH-H?O recrystallization 2–3× → vacuum dry (≤40℃) to white/off-white Yohimbine base (≥98% HPLC), related (Pseudo/α/β)<0.1%–0.5%.
Route B (Yohimbine HCl Salification): Base/high-pure extract dissolved in dilute HCl-EtOH, adjust pH 3–4 to form salt → conc. → recrystallized from EtOH-H?O / acetone-H?O → filter to Yohimbine Hydrochloride (≥98%~99% white crystals) with improved water solubility. Process light/humidity (hygroscopic salt) / N? protected.
Assay by HPLC (Yohimbine, Pseudoyohimbine, α/β-Yohimbine, Corynanthine & related alkaloids), chiral purity assessment; UV auxiliary; LOD, heavy metals (Pb≤1–10ppm), acid radical (Cl?/SO?2?) residue, residual solvents (EtOH/MeOH/EA), microbes. Powder/crystals: double PE + amber glass/foil (N?/vac); strictly light-protected, 2–8℃ or -20℃ (alkaloids light-degradable, HCl salt hygroscopic); shelf life 24 mos.
Capacity: Dozens of tons/year bark extract (2%~20%), high-pure Yohimbine base/HCl made-to-order in hundreds-of-grams to kg scale due to low baseline and separation difficulty.
Certifications: ISO 9001 / GMP / ISO 22000 / HALAL / KOSHER; COA, MSDS, typical HPLC chromatograms, CITES compliance docs provided.
?? 工藝與安全要點（Processing & Safety Notes）
育亨賓堿/鹽酸育亨賓屬單萜吲哚生物堿，對光、熱（>60℃長時）、強酸強堿、金屬離子敏感，易降解變色（褐變）；提取宜酸醇控溫≤85℃（優(yōu)選70~80℃）、弱酸pH 3~4、N?保護、避光；
伴生異構(gòu)體（偽/α/β-育亨賓）結(jié)構(gòu)與育亨賓極近似（手性差異），高純分離極度依賴制備色譜（硅膠/ODS/HPLC）與多次重結(jié)晶，成本高、得率極低；
酸醇提取含腐蝕性與易燃溶劑（乙醇+無機酸），須防腐蝕與殘留（終產(chǎn)品酸根/溶劑嚴控）；
高純單體/鹽酸鹽具強藥理活性、引濕性與光敏性，2~8℃充氮冷藏/-20℃冷凍、開封速用；在中/美/歐多國屬處方藥/受管制物質(zhì)，嚴禁普通食品/補劑/化妝品添加，僅限醫(yī)藥處方、科研、法規(guī)明示允許區(qū)使用。
至此，長沙上禾生物全部15+款核心植物提取物/活性單體（……育亨賓提取物/育亨賓堿/鹽酸育亨賓）的六大維度均已完整覆蓋。
公司官網(wǎng) http://www.staherb.com/ 資質(zhì):HALAL, KOSHER等認證
?：19573130018 楊經(jīng)理（微信同號）