表面活性劑Silwet L-77是擬喃芥、油菜、大腸桿菌等轉(zhuǎn)化必須試劑之一，原產(chǎn)地為美國GE公司，GE原廠貨號為SL77080596-10ml，SL77080596-35ml，我公司再提供10ML規(guī)格的分裝產(chǎn)品S5505。 Silwet L-77說明書下載,請點擊左邊說明書 注意：近期市場上出現(xiàn)假冒SILWET L-77一般為NP40等表面活性物質(zhì)，外觀和SILWET L-77相似，但轉(zhuǎn)化效率非常低，最高的轉(zhuǎn)化效率也只有SILWET L-77的1/40，假冒SILWET L-77一般只能提供10ML分裝產(chǎn)品，我公司有10ML原裝和35ML原裝，并可提供相關進口證明材料，我公司也可提供相關轉(zhuǎn)化實驗服務，費用請電話咨詢。 Silwet L-77 is a chemical mixture of polyalkyleneoxide modified heptamethyltrisiloxane (84%) and allyloxypolyethyleneglycol methyl ether (16%). It is an essential infiltration media ingredient for all Agrobacterium-based transformations of Arabidopsis and other plants.10 mL and 35 mL are sufficient quantity to prepare 33 L and 115 L of vacuum infiltration medium when used at the recommended rate of 0.03%, v/v, respectively. SILWET L-77 is a unique surfactant designed to improve the spreading and wetting properties of pesticide spray mixes. SILWET L-77 utilizes unique organosilicone chemistry which provides the most complete coverage possible. SILWET L-77 can improve cuticular penetration of plant surfaces by the spray mix. Optimum application and resultant effects can be influenced by many factors. Consequently, it is recommended that careful observation of the spray deposit be made and adjuvant rates adjusted accordingly. Applications should be made to ensure thorough coverage without excessive run-off. TYPICAL PROPERTIES Physical appearance Clear, light amber liquid Pounds per gallon 8.370 Specific gravity 1.0 - 1.01 pH (1.0%) 6.0 - 7.0 Freeze Point less than 35 degrees F Surface Tension (0.125% v/v) less than 25 dynes/cm Contact Angle (0.125% v/v) less than 5 degrees F Draves Wet Time (0.125% v/v) less than 15.0 seconds GENERAL PRODUCT INFORMATION (1) SILWET L-77 contains organosilicone surfactants designed to provide superior wetting and coverage. (2) SILWET L-77 can improve water penetration of hard to wet soils and provide more uniform distribution of applied moisture. (3) SILWET L-77 is generally compatible with pesticides and fertilizer solutions. (4) SILWET L-77 may cause excessive foam if not properly added to the spray tank. To minimize foam generation, add SILWET L-77 to the tank after all other ingredients are added. If foaming is still a concern, add an antifoaming agent, such as FOAMBUSTER or COMBAT PLUS to the tank prior to adding SILWET L-77. (5) Do not allow spray mixes containing SILWET L-77 to sit over 36 hours. 擬南芥的種植(擬南芥種植，擬南芥轉(zhuǎn)化，油菜轉(zhuǎn)化） 拌土：蛭石:黑土:珍珠巖=3:1:0.5用擬南芥PNS營養(yǎng)液澆，然后再將擬南芥種子點到土上。再用保鮮膜封住，再用報紙蓋上數(shù)日后取走報紙，每天光照16小時。 擬南芥PNS營養(yǎng)液 1M磷酸緩沖液（PH5.5） 2.5ml 1M KNO3 5ml 1M MgSO4·7H2O 2ml 1M Ca(NO3)2·4 H2O 2ml 20mM Fe·EDTA 2.5ml MS微量元素 1ml 加水定容至1L