PE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay) (Site-specific conjugation)
分子別名（Synonym）
SIGLEC10,MGC126774,PRO940,Siglec10,SLG2
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
PE-Labeled Human Siglec-10, His Tag (SI0-HP2E5) is produced via site-specific conjugation of PE to Human Siglec-10, His Tag under optimal conditions with a proprietary technology with a proprietary technology. Human Siglec-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Predicted N-terminus: Met 17
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.8 kDa.
偶聯(lián)（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
應(yīng)用說明（Application）
Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.
Designed and validated to use together with CD24 overexpressing HEK293 cells.
Not suitable to use together with overexpressing CHO cells for high non-specific background from proteins of high sialylation.
Not recommended and validated to use together with tumor cells.
制劑（Formulation）
Supplied as 0.2 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
運(yùn)輸（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存儲(chǔ)（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.
背景（Background）
Siglecs（唾液酸結(jié)合Ig樣凝集素）是具有粘附分子樣結(jié)構(gòu)的Ig超家族的一個(gè)獨(dú)特子集。我們在這里描述了siglec蛋白家族的一個(gè)新成員，它具有相似的結(jié)構(gòu)，包括五個(gè)Ig樣結(jié)構(gòu)域、一個(gè)跨膜結(jié)構(gòu)域和一個(gè)含有兩個(gè)ITIM信號基序的細(xì)胞質(zhì)尾部。Siglec-10是通過哮喘嗜酸性粒細(xì)胞EST庫的數(shù)據(jù)庫挖掘鑒定的。Siglec-10-VAP-1相互作用似乎介導(dǎo)淋巴細(xì)胞與內(nèi)皮細(xì)胞的粘附，并有可能通過酶終產(chǎn)物改變炎癥微環(huán)境。
關(guān)鍵字： Siglec-10;Siglec-10蛋白;Siglec-10重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。