| 名稱 | Emodin |
| 描述 | Emodin (Frangula emodin) is an 11β-HSD1 inhibitor with a selective IC50 of 186 and 86 nM against 11β-HSD1 in humans and mice, respectively. Emodin has antiviral, anti-inflammatory, and anti-cancer effects. Emodin can improve metabolic disorders in diet-induced obese mice. |
| 細(xì)胞實驗 | Emodin is prepared in DMSO and stored, and then diluted with appropriate medium before use[3]. The T24 human bladder cancer cells, the HCV-29 normal bladder epithelial cells and J82 human bladder cancer cells are are cultured in RPMI 1640 medium supplemented with 10 % fetal bovine serum at 37°C in a humidified atmosphere containing 5 % CO2. Cells are seeded in 96-well plates with 2×104 cells per well. The cells are incubated with Emodin for 24 h at different concentrations (0, 5, 10, 20, 30, 40, 50, 60, 70 μM) and chose the critical concentration (20 μM) treated with cells for 0, 6, 12, 24, 48, 72, 96 h. The cells are incubated with cisplatin for 24 h at different concentrations (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 μg/mL). MTT assay is used to analyze the cell viability. Cells are treated with drugs for 24 h and apoptotic rates are assessed with flow cytometry using AnnexinV-fluorescein isothiocyanate (AnnexinV-FITC)/propidium iodide (PI) kit. Samples are prepared according to the manufacturer's instruction and analyzed by a flow cytometry (FCM) Calibur[3]. |
| 體外活性 | 方法：人A375細(xì)胞用Emodin處理24小時，使用MTT方法檢測細(xì)胞生長抑制情況。
結(jié)果：Emodin抑制A375細(xì)胞生長（IC50=44.91 μM）。[1]
方法：人A549細(xì)胞用Emodin處理72小時，使用MTT方法檢測細(xì)胞生長抑制情況。
結(jié)果：Emodin抑制A549細(xì)胞生長（IC50=28 μM）。[2]
方法：小鼠BALB/3T3細(xì)胞用Emodin處理48小時，使用Alamar藍(lán)方法檢測細(xì)胞生長抑制情況。
結(jié)果：Emodin抑制BALB/3T3細(xì)胞細(xì)胞生長（IC50=32 μM）。[3] |
| 體內(nèi)活性 | 方法：為研究Emodin對小鼠11β-HSD1活性的影響，將Emodin（100 mg/kg、200 mg/kg）單次口服給正常C57BL/6J雄性小鼠。
結(jié)果：Emodin抑制正常C57BL/6J雄性小鼠11β-HSD1活性。[4]
方法：為研究Emodin對肥胖小鼠代謝的影響，將Emodin（100 mg/kg）口服給肥胖小鼠，每日2次。
結(jié)果：Emodin 改善飲食誘導(dǎo)的肥胖 (DIO) 小鼠的胰島素敏感性和脂質(zhì)代謝，降低血糖和肝臟 PEPCK 以及葡萄糖-6-磷酸酶 mRNA。[4] |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | Acetone : 7.14 mg/mL (26.42 mM), Sonication is recommended.
DMSO : 15.3 mg/mL (56.62 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 3 mg/mL (11.1 mM), Sonication is recommended.
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| 關(guān)鍵字 | Spike | SARS-CoV | SARSCoV | SARS | resistance | insulin | Emodin | diabete | coronavirus | CK2 | CaseinKinase | Casein Kinase | casein | Autophagy | 11β-HSD1 |
| 相關(guān)產(chǎn)品 | Guanidine hydrochloride | Naringin | Aceglutamide | Alginic acid | Casein | Hemin | Hydroxychloroquine | Sildenafil citrate | Stavudine | Tamoxifen | Adenosine | Paeonol |
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