Mouse IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free

分子別名（Synonym）

IL-23 alpha & IL-12 beta

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-M52W7) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14-1 (IL23A) & P43432-1 (IL12B)).

Predicted N-terminus: His (IL23A) & Met 23 (IL12B)

蛋白結(jié)構(gòu)（Molecular Characterization）

Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 20 kDa (IL-23A) and 45 kDa (IL-12B) respectively due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-23亞基α（IL-23α）可以與IL12B結(jié)合形成IL-23白細(xì)胞介蛋白，這是一種異二聚體細(xì)胞因子，在先天免疫和適應(yīng)性免疫中起作用。IL-23可能與IL-17共同構(gòu)成外周組織對感染的急性反應(yīng)。IL-23與由IL12RB1和IL23R組成的異二聚體受體復(fù)合物結(jié)合，激活Jak-Stat信號(hào)級聯(lián)，刺激記憶而不是幼稚T細(xì)胞，并促進(jìn)促炎細(xì)胞因子的產(chǎn)生。IL-23誘導(dǎo)自身免疫性炎癥，因此可能是自身免疫性炎性疾病的原因，可能對腫瘤發(fā)生很重要。