Human IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子別名（Synonym）
IL-23 alpha & IL-12 beta
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-H52W5) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # Q9NPF7-1 (IL23A) & P29460-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The reducing (R) protein migrates as 23 kDa (IL-23 alpha) & 40-45 kDa (IL-12 beta) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
純度（Purity）
>95% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
白細(xì)胞介素23亞基α（IL-23 alpha）可與IL12B結(jié)合形成IL-23白細(xì)胞介素，該異二聚體細(xì)胞因子在先天性和適應(yīng)性免疫中發(fā)揮作用。IL-23與IL-17共同構(gòu)成外周組織感染的急性應(yīng)答機(jī)制。IL-23通過與IL12RB1/IL23R組成的異二聚體受體復(fù)合物結(jié)合，激活Jak-Stat信號級聯(lián)反應(yīng)，特異性刺激記憶性T細(xì)胞（而非初始T細(xì)胞），并促進(jìn)促炎細(xì)胞因子的產(chǎn)生。該因子可誘導(dǎo)自身免疫性炎癥，可能介導(dǎo)自身免疫性炎癥疾病的發(fā)生，并在腫瘤形成過程中發(fā)揮重要作用。
關(guān)鍵字： IL23A & IL12B;IL23A蛋白;IL12B重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。