化合物 Histone Deacetylase Inhibitor III,M344

化合物 Histone Deacetylase Inhibitor III|T2157|TargetMol

價(jià)格	￥272	￥618	￥981
包裝	1mg	5mg	10mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2026-04-02

QQ交談微信洽談

產(chǎn)品詳情

中文名稱:化合物 Histone Deacetylase Inhibitor III	英文名稱:M344
CAS:251456-60-7	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
純度規(guī)格: 99.95%	產(chǎn)品類(lèi)別: 抑制劑
貨號(hào): T2157

Product Introduction

Bioactivity

名稱	M344
描述	M344 (Histone Deacetylase Inhibitor III) is a potent HDAC inhibitor with IC50 of 100 nM and able to induce cell differentiation.
細(xì)胞實(shí)驗(yàn)	MEL DS19 cells (murine erythroleukemia cells) are maintained in D-MEM containing 100 units/mL penicillin G sodium and 100 μg/mL streptomycin sulfate supplemented with 10% fetal bovine serum at 37 °C in a 5% CO2 atmosphere. To test M344 for potential to induce cell differentiation, log-phase cells with a population doubling time of 11−13 hours are used. Serial dilutions of M344 are prepared in 24-well plates using 1 mL of D-MEM/well. If M344 are dissolved in DMSO, control wells contains the same amount of solvent (generally 2 μL/mL of medium). Subsequently, the cell suspension is added to the wells. After 72 hours the experiment is evaluated. Cell numbers are counted using a Casy 1 TTC flow cytometer. The proliferation of treated cells is expressed as percent proliferation in comparison with the solvent control. Differentiated MEL cells accumulate hemoglobin. Therefore, the induction of cell differentiation is determined by benzidine staining according to the literature. To 100 μL of cell suspension is added 10 μL of a 0.4% solution of benzidine in 12% acetic acid containing 2% Water2. Within 5 minutes hemoglobin-containing cells stains blue. Benzidine-positive and -negative cells are counted under the microscope in a hemocytometer, and the percentage of positive cells is calculated. M344 is first tested at 10 μM and 50 μM final concentration. According to activity/toxicity profile, a range of concentrations is chosen for a dose−response analysis. (Only for Reference)
激酶實(shí)驗(yàn)	Enzyme Inhibition : Radioactively labeled chicken core histones are used as the enzyme substrate. The enzyme liberated tritiated acetic acid from the substrate which is quantitated by scintillation counting. IC50 values are results of triple determinations. 50 μL of maize enzyme (at 30 °C) is incubated (30 minutes) with 10 μL of total [3H]acetate-prelabeled chicken reticulocyte histones (1 mg/mL). Reaction is stopped by addition of 36 μL of 1 M HCl/0.4 M acetate and 800 μL of ethyl acetate. After centrifugation (10000 g, 5 minutes) an aliquot of 600 μL of the upper phase is counted for radioactivity in 3 mL of liquid scintillation cocktail. M344 is tested in a starting concentration of 40 μM, and active substances are diluted further.
體內(nèi)活性	在體外,M344顯示出對(duì)子宮內(nèi)膜癌細(xì)胞系Ishikawa和卵巢癌細(xì)胞系SK-OV-3的顯著抗增殖活性,EC50分別為2.3 μM和5.1 μM。而正常的人子宮內(nèi)膜上皮細(xì)胞對(duì)M344幾乎不敏感。高于10 μM濃度時(shí)的M344具有毒性,而最多只有20%的存活細(xì)胞群被誘導(dǎo)分化。此外,M344也會(huì)導(dǎo)致細(xì)胞周期中S期細(xì)胞比例減少,G0/G1期細(xì)胞比例增加,誘導(dǎo)細(xì)胞凋亡,并降低線粒體的跨膜電位。
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
溶解度	Ethanol : 4 mg/mL (13.01 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble) 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (6.51 mM), Sonication is recommended. DMSO : 62 mg/mL (201.7 mM), Sonication is recommended.
關(guān)鍵字	MS-344 \| MS344 \| M-344 \| M344 \| M 344 \| Inhibitor \| inhibit \| Histone deacetylases \| HDAC \| D-237 \| D237 \| D 237
相關(guān)產(chǎn)品	Valproic acid sodium salt \| 4-Phenylbutyric acid \| Valproic Acid \| Sodium butanoate \| Panobinostat \| Manganese chloride (tetrahydrate) \| Methoxyacetic acid \| Theophylline \| Acefylline \| Curcumin \| Theophylline monohydrate \| Sodium 4-phenylbutyrate
相關(guān)庫(kù)	抑制劑庫(kù) \| 經(jīng)典已知活性庫(kù) \| 抗癌化合物庫(kù) \| 已知活性化合物庫(kù) \| 自噬庫(kù) \| 抗胰腺癌化合物庫(kù) \| 抗衰老化合物庫(kù) \| HIF-1化合物庫(kù) \| 表觀遺傳庫(kù) \| 癌細(xì)胞分化化合物庫(kù) \| NO PAINS 化合物庫(kù) \| 細(xì)胞重編程化合物庫(kù)

關(guān)鍵字： MS 344|||Histone Deacetylase Inhibitor III|TargetMol

公司簡(jiǎn)介

TargetMol Chemicals Inc. 總部位于馬薩諸塞州波士頓，致力于為全球生化領(lǐng)域科學(xué)家的研究提供專業(yè)的產(chǎn)品和服務(wù)。TargetMol?品牌的客戶群分布于40多個(gè)國(guó)家和地區(qū)，已發(fā)展成為全球知名的化合物庫(kù)和小分子化合物研究供應(yīng)商。 TargetMol?可提供160多種滿足不同需求的化合物庫(kù)，以及多種類(lèi)型的生化試劑產(chǎn)品，包括12000多種抑制劑、16000多種天然產(chǎn)物和各類(lèi)多肽、抗體、生命科學(xué)試劑盒等，此外，我們還建設(shè)有CADD（計(jì)算機(jī)輔助藥物設(shè)計(jì)）研究中心、藥理實(shí)驗(yàn)室、藥化合成平臺(tái)三大技術(shù)中心，全方位滿足客戶的定制需求。憑借我們優(yōu)質(zhì)的產(chǎn)品和服務(wù)、快速高效的全球供應(yīng)鏈和專業(yè)的技術(shù)支持，我們將有效幫助您縮短研發(fā)周期，取得更成功的結(jié)果。

成立日期	2013-04-18 （14年）	注冊(cè)資本	566.265100萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	天然產(chǎn)物,生化試劑,分子生物學(xué),分子砌塊,生物技術(shù)服務(wù)	經(jīng)營(yíng)模式	貿(mào)易,工廠,試劑,定制,服務(wù)

TargetMol中國(guó)（陶術(shù)生物）

VIP 5年

公司成立：14年
注冊(cè)資本：566.265100萬(wàn)人民幣
企業(yè)類(lèi)型：有限責(zé)任公司(自然人投資或控股)
主營(yíng)產(chǎn)品：小分子抑制劑、藥物篩選化合物庫(kù)、藥物篩選等
公司地址：靜安區(qū)江場(chǎng)三路238號(hào)8樓

進(jìn)入店鋪

詢盤(pán)

店內(nèi)推薦

化合物 Phox-I2|T25952|TargetMol

詢價(jià)

環(huán)噻嗪|T4573|TargetMol

￥219

化合物 RU 24969 free base|T8593|TargetMol

￥552