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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, SKOV3 cells, Jurkat cells, Primary antibody: P01502(KLHL9 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The KLHL9 antibody is a research tool designed to detect and analyze the Kelch-like family member 9 (KLHL9) protein, a component of the ubiquitin-proteasome system. KLHL9 belongs to the BTB-Kelch protein family, characterized by a BTB (BROAD-TRAMPLEX, BR-C, TTB, and POZ) domain for protein interactions and Kelch repeats involved in substrate recognition. It acts as a substrate adaptor for Cullin 3 (CUL3)-based E3 ubiquitin ligase complexes, targeting specific proteins for ubiquitination and subsequent degradation. KLHL9 has been implicated in cellular processes such as cell cycle regulation, cytoskeletal organization, and protein quality control. Dysregulation of KLHL9 is linked to diseases, including certain cancers and neurodegenerative disorders.

Antibodies targeting KLHL9 are typically developed in rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to study KLHL9 expression, localization, and interactions. Specificity is confirmed via knockout controls or siRNA knockdown. Researchers use these antibodies to explore KLHL9's role in disease mechanisms, its interaction partners (e.g., CUL3), and its potential as a therapeutic target. Commercial KLHL9 antibodies often include validation data to ensure reproducibility, though performance may vary depending on experimental conditions.