描述（Description）
IL-1 alpha is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1 alpha mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways[1][4]. IL-1 alpha protein, Human is a recombinant protein consisting of 159 amino acids (S113-A271) and is produced by E. coli.
純度（Purity）
≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining)
生物活性（Bioactivity）
Measured by its ability to induce NF-kB reporter activity by the reporter gene assay. TheED50 for this effect is ≤0.05ng/ml.
氨基酸序列Target Protein Sequence）
SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA
制劑（Trait）
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
復(fù)溶（Reconstitution）
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存條件&效期（Storage & Stability）
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
運輸方式(Shipping)
Room temperature in continental US; may vary elsewhere.
生物活性（Bioactivity）
1.The ED50 is <1.0 pg/mL as measured by murine D10S cells, corresponding to a specific activity of >1.0 × 109 units/mg.
2.Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 10.95 pg/mL, corresponding to a specific activity is 91.32×106 units/mg.
3.Measured by its ability to down regulate the expression of CCN2 in NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20.03-24.05 pg/mL, corresponding to a specific activity is 4.158-4.992×107 U/mg.
Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 10.95 pg/mL，corresponding to a specific activity is 91.32×106 units/mg
電泳（SDS-PAGE）
Greater than 95% as determined by reducing SDS-PAGE.
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關(guān)鍵字： GMP級;高純度;批間一致;生物活性;
在生物科技領(lǐng)域，豐度蛋白專注重組蛋白供應(yīng)，以“豐度的風(fēng)帆為蛋白提供動力，豐度為蛋白護(hù)航”為使命，賦能生命科學(xué)發(fā)展。
蘇州豐度依托先進(jìn)技術(shù)平臺，打造成熟生產(chǎn)體系，嚴(yán)控基因工程設(shè)計、細(xì)胞培養(yǎng)、蛋白純化等環(huán)節(jié)，優(yōu)化工藝與管理，確保產(chǎn)品高純度、高活性且批間一致。產(chǎn)品覆蓋基礎(chǔ)研究、生物醫(yī)藥研發(fā)等多領(lǐng)域，為科研及生產(chǎn)提供多樣、適配產(chǎn)品，助力突破技術(shù)難題 。