Human IL-5 Protein, His Tag

分子別名（Synonym）

IL-5, TRF, IL5, Interleukin-5

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-5 Protein, His Tag (IL5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 15.0 kDa. The protein migrates as 18-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

白細(xì)胞介素5（IL-5）是一種由2型輔助T細(xì)胞和肥大細(xì)胞產(chǎn)生的白細(xì)胞介素。作為造血家族成員的TH2細(xì)胞因子，IL-5在人類中由115個(gè)氨基酸構(gòu)成（小鼠為133個(gè)氨基酸）。與此細(xì)胞因子家族其他成員（即IL-3和GM-CSF）不同，該糖蛋白的活性形式為同源二聚體。

IL-5長期被認(rèn)為與多種過敏性疾病的發(fā)病機(jī)制相關(guān)，包括過敏性鼻炎和哮喘。在這些疾病中可觀察到循環(huán)系統(tǒng)、氣道組織及誘導(dǎo)痰中嗜酸性粒細(xì)胞數(shù)量顯著增加。鑒于嗜酸性粒細(xì)胞與過敏性疾?。ㄌ貏e是過敏性哮喘）病理的高度相關(guān)性，學(xué)界廣泛推測(cè)嗜酸性粒細(xì)胞在該疾病病理過程中起重要作用。靶向IL-5的藥物包括美泊利單抗（mepolizumab）和瑞利珠單抗（reslizumab）。