Biotinylated Human IL-5 Protein, His,Avitag? (MALS verified)
分子別名（Synonym）
IL-5, TRF, IL5, Interleukin-5
表達區(qū)間及表達系統(tǒng)（Source）
Biotinylated Human IL-5, His,Avitag (IL5-H82Q5) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1).
Predicted N-terminus: His
蛋白結構（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag?).
The protein has a calculated MW of 16.7 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
標記（Labeling）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
純度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
白細胞介素5（IL-5）是一種由2型輔助T細胞和肥大細胞產(chǎn)生的白細胞介素。這種由115個氨基酸（人類；小鼠為133個氨基酸）組成的TH2細胞因子屬于造血因子家族。不同于該細胞因子家族的其他成員（即白細胞介素3和GM-CSF），這種糖蛋白的活性形式為同源二聚體。長期以來，白細胞介素5被視為過敏性鼻炎與哮喘等過敏疾病的致病因素，在這些疾病中可觀察到循環(huán)系統(tǒng)、氣道組織及誘導痰中的嗜酸性粒細胞數(shù)量顯著增加。鑒于嗜酸性粒細胞與過敏性疾?。ㄌ貏e是過敏性哮喘）病理機制的高度關聯(lián)性，學界普遍推測該類細胞在該疾病病理過程中起重要作用。靶向IL-5的藥物包括美泊利單抗與瑞利珠單抗。
關鍵字： IL-5;IL-5蛋白;IL-5重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。