一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of Antimicrobial Chemotherapy >>article
Journal of Antimicrobial Chemotherapy

Journal of Antimicrobial Chemotherapy

IF: 3.9
Download PDF

Repurposing cinacalcet suppresses multidrug-resistant Staphylococcus aureus by disruption of cell membrane and inhibits biofilm by targeting IcaR

Published:2 April 2024 DOI: 10.1093/jac/dkae051 PMID: 38412335
Zu-Ye Fang,?Zi-Yuan Zhang,?Yun-Dan Zheng,?Dan Lei,?Jianpeng Zhuang,?Nan Li,?Qing-Yu He,?Xuesong Sun

Abstract

Background: MDR Staphylococcus aureus infections, along with the severity of biofilm-associated infections, continue to threaten human health to a great extent. It necessitates the urgent development of novel antimicrobial and antibiofilm agents.

Objectives: To reveal the mechanism and target of cinacalcet as an antibacterial and antimicrobial agent for S. aureus.

Methods: Screening of non-antibiotic drugs for antibacterial and antibiofilm properties was conducted using a small-molecule drug library. In vivo efficacy was assessed through animal models, and the antibacterial mechanism was studied using quantitative proteomics, biochemical assays, LiP-SMap, BLI detection and gene knockout techniques.

Results: Cinacalcet, an FDA-approved drug, demonstrated antibacterial and antibiofilm activity against S. aureus, with less observed development of bacterial resistance. Importantly, cinacalcet significantly improved survival in a pneumonia model and bacterial clearance in a biofilm infection model. Moreover, the antibacterial mechanism of cinacalcet mainly involves the destruction of membrane-targeted structures, alteration of energy metabolism, and production of reactive oxygen species (ROS). Cinacalcet was found to target IcaR, inhibiting biofilm formation through the negative regulation of IcaADBC.

Conclusions: The findings suggest that cinacalcet has potential for repurposing as a therapeutic agent for MDR S. aureus infections and associated biofilms, warranting further investigation.

Similar articles

IF:0

Bioactivity of Mupirocin Nanoparticle-Loaded Hydrogel against Methicillin-Resistant Staphylococcus aureus (MRSA)

Applied microbiology Sukanjana Kamlungmak,?Monwadee Wonglapsuwan,etc Published: 3 March 2022
霍州市| 广平县| 敦煌市| 秭归县| 广宁县| 太康县| 永济市| 浙江省| 富源县| 乌苏市| 龙江县| 闵行区| 遵义市| 同仁县| 金昌市| 甘南县| 长乐市| 奎屯市| 南投市| 县级市| 信丰县| 惠来县| 于都县| 封开县| 西林县| 太湖县| 吴桥县| 剑河县| 濮阳县| 芜湖县| 四子王旗| 宿州市| 新郑市| 榆社县| 南乐县| 临海市| 宝鸡市| 黑山县| 萨嘎县| 双鸭山市| 甘孜|