一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Heliyon >>article

Heliyon

IF: 3.4
Download PDF

Reprogramming human urine cells into intestinal organoids with long-term expansion ability and barrier function

Published:27 June 2024 DOI: 10.1016/j.heliyon.2024.e33736 PMID: 39040281
Ruifang Zhang,?Yating Chen,?Ziyu Feng,?Baomei Cai,?Yiyi Cheng,?Yunjing Du,?Sihua Ou,?Huan Chen,?Mengjie Pan,?He Liu,?Duanqing Pei,?Shangtao Cao

Abstract

Generation of intestinal organoids from human somatic cells by reprogramming would enable intestinal regeneration, disease modeling, and drug screening in a personalized pattern. Here, we report a direct reprogramming protocol for the generation of human urine cells induced intestinal organoids (U-iIOs) under a defined medium. U-iIOs expressed multiple intestinal specific genes and showed resembling gene expression profiles to primary small intestines. U-iIOs can be stably long-term expanded and further differentiated into more mature intestinal lineage cells with high expression of metallothionein and cytochrome P450 (CYP450) genes. These specific molecular features of U-iIOs differ from human pluripotent stem cells derived intestinal organoids (P-iIOs) and intestinal immortalized cell lines. Furthermore, U-iIOs exhibit intestinal barriers indicated by blocking FITC-dextran permeation and uptaking of the specific substrate rhodamine 123. Our study provides a novel platform for patient-specific intestinal organoid generation, which may lead to precision treatment of intestinal diseases and facilitate drug discovery.

Substances (12)

Materials
Procduct Name CAS Molecular Formula Supplier Price
CHIR-99021 252917-06-9 C22H18Cl2N8 342 suppliers $28.00-$4401.00
CHIR-99021 252917-06-9 C22H18Cl2N8 342 suppliers $28.00-$4401.00
CHIR-99021 252917-06-9 C22H18Cl2N8 342 suppliers $28.00-$4401.00
CHIR-99021 252917-06-9 C22H18Cl2N8 342 suppliers $28.00-$4401.00
RG108 48208-26-0 C19H14N2O4 184 suppliers $20.00-$2416.00
RG108 48208-26-0 C19H14N2O4 184 suppliers $20.00-$2416.00
RG108 48208-26-0 C19H14N2O4 184 suppliers $20.00-$2416.00
RG108 48208-26-0 C19H14N2O4 184 suppliers $20.00-$2416.00
EPZ011969 1598383-40-4 C35H51N5O4 92 suppliers $24.00-$1932.00
EPZ011969 1598383-40-4 C35H51N5O4 92 suppliers $24.00-$1932.00

Similar articles

IF:0

Long-term toxicity of sodium cyclamate in mice

Food and cosmetics toxicology P.G Brantom , I.F Gaunt ,etc Published: 1 October 1973
IF:6.6

Long-term safety and efficacy of lacosamide and controlled-release carbamazepine monotherapy in patients with newly diagnosed epilepsy

Epilepsia Elinor Ben-Menachem, Hans Peter Grebe,etc Published: 21 November 2019
IF:2.1

Effect of acute and long term potassium bromide administration on spatial working memory in rat.

Research in Pharmaceutical Sciences Faezeh Safdari, Mohammad Rabbani,etc Published: 1 April 2017
太保市| 康定县| 克山县| 黔东| 兴隆县| 铜梁县| 永新县| 揭西县| 乐安县| 乌拉特后旗| 隆昌县| 塔河县| 呼伦贝尔市| 托克托县| 玉溪市| 潜山县| 乌兰县| 青岛市| 沐川县| 霞浦县| 云梦县| 会同县| 天全县| 青海省| 含山县| 广汉市| 平武县| 五指山市| 阳信县| 库尔勒市| 靖边县| 九江市| 安义县| 全州县| 沙洋县| 河池市| 登封市| 巴彦淖尔市| 靖安县| 布拖县| 梓潼县|