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Applied Spectroscopy

Applied Spectroscopy

IF: 2.2
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Using Label-Free Raman Spectroscopy Integrated with Microfluidic Chips to Probe Ferroptosis Networks in Cells

Published:1 July 2025 DOI: 10.1177/00037028241292087 PMID: 39529267
Muhammad Muhammad,?Chang-Sheng Shao,?Raziq Nawaz,?Amil Aligayev,?Muhammad Hassan,?Mona Alrasheed Bashir,?Jamshed Iqbal,?Jie Zhan,?Qing Huang

Abstract

Ferroptosis, a regulated form of cell death driven by oxidative stress and lipid peroxidation, has emerged as a pivotal research focus with implications across various cellular contexts. In this study, we employed a multifaceted approach, integrating label-free Raman spectroscopy and microfluidics to study the mechanisms underpinning ferroptosis. Our investigations included the ferroptosis initiation based on the changes in the lipid Raman band at 1436 cm-1 under different cellular states, the generation of reactive oxygen species (ROS), lipid peroxidation, DNA damage/repair, and mitochondrial dysfunction. Importantly, our work highlighted the dynamic role of vital cellular components, such as nicotinamide adenine dinucleotide phosphate hydrogen (NADPH), ferredoxin clusters, and other key factors such as glutathione peroxidase 4 and nuclear factor erythroid 2, which collectively influenced cellular responses to redox imbalance and oxidative stress. Quantum mechanical (QM) and molecular docking simulations (MD) provided further evidence of interactions between the ferredoxin (containing 4Fe-4S clusters), NADPH, and ROS, which led to the production of reactive Fe species in the cells. As such, our approach not only offered a real-time, multidimensional perspective on ferroptosis but also provided valuable methods and insights for therapeutic interventions in diverse biomedical contexts.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Hoechst 33342 23491-52-3 C27H28N6O 369 suppliers $18.00-$2370.00
Hoechst 33342 23491-52-3 C27H28N6O 369 suppliers $18.00-$2370.00
Hoechst 33342 23491-52-3 C27H28N6O 369 suppliers $18.00-$2370.00
Hoechst 33342 23491-52-3 C27H28N6O 369 suppliers $18.00-$2370.00

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