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Food Chemistry

Food Chemistry

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A rapid and sensitive sandwich assay for the detection of Alicyclobacillus acidoterrestris in apple juice based on magnetosome immunomagnetic separation combined with quantum dots immunoassay technology

Published:26 November 2024 DOI: 10.1016/j.foodchem.2024.142234
Jianrui Qi, Kai Wang, Tianli Yue

Abstract

The contamination of apple juice by Alicyclobacillus acidoterrestris (A. acidoterrestris) can cause significant economic losses. Therefore, developing a rapid and sensitive method for detecting A. acidoterrestris is necessary. To address this issue, this study prepared magnetosome–monoclonal antibodies immunomagnetic microspheres (IMMs) as capture probes to enrich A. acidoterrestris (IMMs-Aa) and monoclonal antibodies-quantum dots (mAb-CdTeQDs) as detection probes for detecting A. acidoterrestris. The obtained IMMs-Aa-mAb-CdTeQDs “sandwich” structure was used to detect A. acidoterrestris based on the fluorescence intensity. The strategy presented a good linear correlation (y?=?1048×?+?1891, R2?=?0.995) between the fluorescence intensity and the concentration of A. acidoterrestris (101–105?CFU/mL) with a low detection limit of 25.4?CFU/mL within 50?min. The recovery rate of this strategy in spiked apple juice ranged from 88.16?% to 107.03?%. Thus, this study established an efficient and highly sensitive magnetic capture-enrichment-separation-detection method for A. acidoterrestris.

Substances (10)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Citric acid 77-92-9 C6H8O7 2073 suppliers $5.00-$2290.00
Glutathione 70-18-8 C10H17N3O6S 1413 suppliers $6.00-$3580.00
Calcium chloride 10043-52-4 CaCl2 1213 suppliers $5.00-$26371.00
Potassium Phosphate Monobasic 7778-77-0 H3O4P.K 1180 suppliers $5.00-$8420.00
Ammonium sulfate 7783-20-2 H8N2O4S 976 suppliers $9.00-$5423.00
Bovine albumin 9048-46-8 N/A 915 suppliers $15.00-$18680.00
Magnesium sulfate heptahydrate 10034-99-8 Mg.O4S.7H2O 825 suppliers $10.00-$7150.00
Yeast extract 8013-01-2 n.a. 578 suppliers $34.70-$8930.00
Cadmium chloride hydrate 7790-78-5 CdCl2H2O 181 suppliers $17.00-$1440.26
GLUCOSE 58367-01-4 C6H12O6 167 suppliers Inquiry

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