Cold-water fish cell-cultured meat production presents a unique challenge due to the significantly low culture temperature required for cell growth, where bioinks for cold bioprinting are designed with suitable rheological properties to support cell viability. Here, we develop a novel composite hydrogel bioink composed of warm-water fish gelatin (FG) and cold-water fish gelatin (CFG) optimized for low-temperature (<18?°C) 3D bioprinting (LT-bio3DP) of rainbow trout muscle satellite cells (rtMSCs). The bioinks are systematically evaluated for their structural, rheological, and biological properties. The results demonstrate that gelation temperature decreases from 20.11?°C to 2.33?°C as increasing CFG. The followed reduction of storage modulus and viscosity (while increase of hydrophilicity) influence the printability and cell behavior. The 4FG6CFG hydrogel is identified as the optimal composition of LT-bio3DP, with balanced structural stability, extrusion performance, and cell compatibility. It promotes rtMSCs proliferation and myogenic differentiation successfully. Printed scaffolds maintain their morphology during long-term culture, and exhibited textural hardness comparable to native rainbow trout meat tissue. Overall, we provide an innovative strategy for developing cold-temperature bioprinting and biomaterials tailored to cell cultivation.