一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Oncology Research >>article
Oncology Research

Oncology Research

IF: 4.1
Download PDF

OTUB2 promotes proliferation and metastasis of triple-negative breast cancer by deubiquitinating TRAF6

Published:18 April 2025 DOI: 10.32604/or.2025.062767 PMID: 40296903
Y U Qiu,?Ruihan Liu,?Shanshan Huang,?Qiaoting Cai,?Y I Xie,?Zhiting He,?Weige Tan,?Xinhua Xie

Abstract

Objectives: Deubiquitinase OTUB2 plays a critical role in the progression of various tumors. However, its specific role in triple-negative breast cancer (TNBC) remains unclear. This study aims to elucidate the biological function of OTUB2 in TNBC and uncover the underlying mechanisms.

Methods: First, we found that the expression of OTUB2 was upregulated in TNBC by bioinformatics analysis, we then validated its expression in TNBC tissues and cells using immunohistochemistry (IHC) and qPCR and plotted the survival curves by Kaplan-Meier method. Gene set enrichment analysis (GSEA) suggested that OTUB2 may be involved in tumor proliferation and metastasis. Further functional assays, including Cell Counting Kit-8 (CCK-8), colony formation, Transwell, and wound healing assays, were performed to assess the effects of OTUB2 overexpression and knockdown on TNBC cell proliferation and migration. Additionally, UbiBrowser 2.0 was used to identify OTUB2 substrate proteins and western blotting was conducted to clarify the molecular mechanisms involved.

Results: Our results demonstrated that OTUB2 expression was elevated in TNBC and associated with poor prognosis. Overexpression of OTUB2 enhanced the proliferation and migration of TNBC cells, while its knockdown inhibited these processes. Moreover, OTUB2 stabilized tumor necrosis factor receptor-associated factor 6 (TRAF6) by deubiquitinating it, leading to activation of the protein kinase B (AKT) pathway.

Conclusions: OTUB2 exerts its promoting effects on the progression of TNBC by activating the TRAF6/AKT pathway.

Similar articles

IF:4.6

Targeting cuproptosis by zinc pyrithione in triple-negative breast cancer.

iScience Xu Yang,?Li Deng,etc Published: 16 October 2023
IF:10.5

Self-assembling mertansine prodrug improves tolerability and efficacy of chemotherapy against metastatic triple-negative breast cancer

Journal of Controlled Release Wei Ran , Xiaoyu Liu ,etc Published: 1 February 2020
IF:10

Hybrid Cell Membrane‐Coated Nanoparticles for Synergizing Sonodynamic Therapy and Immunotherapy against Triple‐Negative Breast Cancer

Advanced Healthcare Materials Jiali Gong, Danling Cheng,etc Published: 21 November 2024
德州市| 凤台县| 怀柔区| 金沙县| 安多县| 临西县| 卢湾区| 泸定县| 勐海县| 昌平区| 封丘县| 南平市| 咸丰县| 崇左市| 伊川县| 民乐县| 威海市| 淮阳县| 普洱| 闽清县| 东山县| 肇东市| 淮南市| 秦安县| 梁平县| 龙山县| 和平区| 嘉禾县| 宜阳县| 临安市| 威远县| 丹凤县| 资溪县| 左贡县| 青神县| 南江县| 抚宁县| 蓬溪县| 丹棱县| 旬邑县| 周宁县|