一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Cell reports >>article
Cell reports

Cell reports

IF: 7.5
Download PDF

Structural basis of peptide recognition and modulation for neuropeptide FF receptors

Published:23 September 2025 DOI: 10.1016/j.celrep.2025.116160 PMID: 40839429
Xinzhu Li,?Heng Zhang,?Wen Hu,?Kai Wu,?Shuai Li,?Sanshan Jin,?Yuling Yin,?Qingning Yuan,?H Eric Xu,?Benxun Pan,?Yi Jiang

Abstract

Neuropeptide FF receptors 1 and 2 (NPFFR1 and NPFFR2) are RF-amide peptide receptors that couple to Gi/o proteins and regulate pain, opioid tolerance, and metabolism. Despite their physiological significance, their ligand selectivity and activation mechanisms remain unclear. Using cryoelectron microscopy, we resolved four NPFFR1 and NPFFR2 structures bound to NPFF or NPVF, revealing conserved C-terminal RF-amide interactions within the orthosteric pocket and N-terminal variations driving subtype specificity. Structural and mutagenesis analyses identified ECL2 and the receptor N terminus as key determinants of NPVF-NPFFR1 and NPFF-NPFFR2 selectivity. Additionally, the structures elucidate the activation mechanism and uncover distinct Gi-coupling features between NPFFR subtypes. These findings provide molecular insights into peptide recognition and receptor activation within the RF-amide family, offering a structural framework for designing selective NPFFR modulators to treat pain, addiction, and metabolic disorders with enhanced specificity and reduced off-target effects.

Similar articles

IF:2.2

Benzimidazole drugs and modulation of biotransformation enzymes

Research in veterinary science J Vel??k , V Baliharová ,etc Published: 1 April 2004
IF:4.3

Discovery of brevijanazines from Aspergillus brevijanus reveals the molecular basis for p-nitrobenzoic acid in fungi?

Chemical Communications Hang Li , Paul M. Mirzayans ,etc Published: 1 January 2022
IF:3.2

β-Galactosidase: Immune recognition of conformation and mechanism of antibody-induced catalytic activation

Biopolymers Franco Celada, Roberto Strom,etc Published: 1 January 1983
南平市| 江川县| 磴口县| 广安市| 广饶县| 唐河县| 大埔县| 宁都县| 宾川县| 抚松县| 兴业县| 涿州市| 安西县| 普陀区| 八宿县| 桐乡市| 巴中市| 锡林浩特市| 玉屏| 辉县市| 德令哈市| 衡阳县| 金川县| 元朗区| 秀山| 交口县| 石城县| 宜丰县| 连云港市| 衡山县| 上饶市| 永城市| 集贤县| 玉山县| 利辛县| 太和县| 健康| 富民县| 白朗县| 临朐县| 女性|