一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Toxicology and applied pharmacology >>article
Toxicology and applied pharmacology

Toxicology and applied pharmacology

IF: 3.3
Download PDF

10-Hydroxy-2-decenoic acid ameliorates LPS-induced acute lung injury through targeting MD2-mediated inflammatory signaling pathways

Published:4 October 2025 DOI: 10.1016/j.taap.2025.117589 PMID: 41052684
Cong Shu , Chen Li , Huamin Liu , Zengxian Zhou , Chenghao Qian , Zhuohui Xie , Fenfen Lin , Yuxiao Jiang , Ruping Chen , Yue Liu , Xin Liu , Gaozhi Chen , Lili Fu

Abstract

Background

Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome (ARDS), are life-threatening conditions with high mortality, characterized by excessive inflammatory responses. Lipopolysaccharide (LPS) is widely used to mimic ALI by activating myeloid differentiation factor 2 (MD2)/Toll-like receptor 4 (TLR4)-mediated inflammatory pathways. Royal jelly-derived 10-hydroxy-2-decenoic acid (10-HDA) exhibits anti-inflammatory properties, but its role in ALI remains unexplored.

Objective

This study aimed to investigate the therapeutic potential of 10-HDA against LPS-induced ALI and elucidate its underlying mechanism.

Methods

In vitro, mouse peritoneal macrophages (MPMs) were pretreated with 10-HDA before LPS stimulation. In vivo, ALI was induced in mice via intratracheal LPS, with 10-HDA administered intraperitoneally. Cytokine levels were measured via ELISA and qPCR. Signaling pathways were analyzed by Western blot and immunofluorescence. Lung injury, inflammatory cell infiltration, and edema were assessed via histopathology, BALF analysis, and wet/dry ratio. Immunoprecipitation, molecular docking, and drug affinity-responsive target stability (DARTS) assays were used to identify the interaction between 10-HDA and MD2.

Results

10-HDA significantly suppressed LPS-induced proinflammatory cytokine secretion in MPMs and ALI mice, and inhibited phosphorylation of TAK1, MAPKs, TBK1 and NF-κB nuclear translocation. It attenuated lung histopathological damage, neutrophil infiltration, and edema. Mechanistically, 10-HDA disrupted MD2/TLR4-mediated inflammatory pathways by directly binding MD2, as confirmed by immunoprecipitation, DARTS, and molecular docking.

Conclusion

10-HDA alleviates LPS-induced ALI by targeting MD2 to block TLR4 signaling, thereby suppressing both MyD88-dependent (MAPKs/NF-κB) and TRIF-dependent (TBK1/IRF3) pathways. These findings highlight 10-HDA as a promising therapeutic candidate for ALI/ARDS.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
10-Hydroxy-2-decenoic acid 765-01-5 C10H18O3 162 suppliers Inquiry
10-Hydroxy-2-decenoic acid 765-01-5 C10H18O3 162 suppliers Inquiry
10-Hydroxy-2-decenoic acid 765-01-5 C10H18O3 162 suppliers Inquiry
10-Hydroxy-2-decenoic acid 765-01-5 C10H18O3 162 suppliers Inquiry

Similar articles

IF:5.3

HBSP inhibits tubular cell pyroptosis and apoptosis, promotes macrophage M2 polarization, and protects LPS‐induced acute kidney injury

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Lili Huang, Yuanyuan Wu,etc Published: 25 November 2024
IF:7.1

Itaconic acid ameliorates necrotizing enterocolitis through the TFEB-mediated autophagy-lysosomal pathway

Free Radical Biology and Medicine Baozhu Chen, Yufeng Liu,etc Published: 19 November 2024
宁远县| 历史| 黄山市| 阿拉善右旗| 昆山市| 敦煌市| 葫芦岛市| 印江| 新昌县| 西充县| 喀喇沁旗| 上高县| 普格县| 元朗区| 水富县| 曲松县| 合作市| 望城县| 安泽县| 元江| 女性| 乌拉特后旗| 四平市| 徐水县| 潮州市| 满城县| 池州市| 洛浦县| 青海省| 公主岭市| 班戈县| 双峰县| 贺兰县| 乌拉特前旗| 津市市| 平阳县| 察雅县| 珲春市| 金川县| 临潭县| 敖汉旗|