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Hormone and Metabolic Research

Hormone and Metabolic Research

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Regulation of phosphatidylcholine biosynthesis by the methylation pathway in rat pituitary gland.

Published:1 August 1984 DOI: 10.1055/s-2007-1014809 PMID: 6469209
C Prasad, R M Edwards

Abstract

Rat pituitary extracts catalyze methylation of phosphatidylethanolamine to phosphatidylcholine using S-adenosyl-L-methionine as the methyl donor. In vitro incubation of hemipituitaries with 1 mM 2-methylaminoethanol led to a dose dependent decrease in phosphatidylethanolamine methyltransferase activity as well as the incorporation of the radioactivity from [3H-methyl]-L-methionine into phosphatidyl-choline. The inhibitory effect of 2-methylaminoethanol was selective for phospholipids methylation since protein carboxymethyltransferase and catechol-o-methyltransferase activities were not affected.

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