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Journal of Chromatography B

Journal of Chromatography B

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Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

Published:15 April 2018 DOI: 10.1016/j.jchromb.2018.03.021 PMID: 29550683
Yan Wu , Jin Yang , Cailing Duan , Liuxi Chu , Shenghuo Chen , Shan Qiao , Xiaoming Li , Huihua Deng

Abstract

The determination of the concentrations of antiretroviral drugs in hair is believed to be an important means for the assessment of the long-term adherence to highly active antiretroviral therapy. At present, the combination of tenofovir, lamivudine and nevirapine is widely used in China. However, there was no research reporting simultaneous determination of the three drugs in hair. The present study aimed to develop a sensitive method for simultaneous determination of the three drugs in 2-mg and 10-mg natural hair (Method 1 and Method 2). Hair samples were incubated in methanol at 37?°C for 16?h after being rinsed with methanol twice. The analysis was performed on high performance liquid chromatography tandem mass spectrometry with electronic spray ionization in positive mode and multiple reactions monitoring. Method 1 and Method 2 showed the limits of detection at 160 and 30?pg/mg for tenofovir, at 5 and 6?pg/mg for lamivudine and at 15 and 3?pg/mg for nevirapine. The two methods showed good linearity with the square of correlation coefficient >0.99 at the ranges of 416–5000 and 77–5000?pg/mg for tenofovir, 12–5000 and 15–5000?pg/mg for lamivudine and 39–50,000 and 6–50,000?pg/mg for nevirapine. They gave intra-day and inter-day coefficient of variation <15% and the recoveries ranging from 80.6 to 122.3% and from 83.1 to 114.4%. Method 2 showed LOD and LOQ better than Method 1 for tenofovir and nevirapine and matched Method 1 for lamivudine, but there was high consistency between them in the determination of the three drugs in hair. The population analysis with Method 2 revealed that the concentrations in hair were decreased with the distance of hair segment away from the scalp for the three antiretroviral drugs.

Substances (20)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Tenofovir 147127-20-6 C9H14N5O4P 694 suppliers $5.00-$1710.00
Tenofovir 147127-20-6 C9H14N5O4P 694 suppliers $5.00-$1710.00
Tenofovir 147127-20-6 C9H14N5O4P 694 suppliers $5.00-$1710.00
Tenofovir 147127-20-6 C9H14N5O4P 694 suppliers $5.00-$1710.00
Nevirapine 129618-40-2 C15H14N4O 403 suppliers $5.00-$1891.00
Nevirapine 129618-40-2 C15H14N4O 403 suppliers $5.00-$1891.00
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