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Journal of Chromatography B

Journal of Chromatography B

IF: 2.8
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Bioanalytical assay for the quantification of the ALK inhibitor lorlatinib in mouse plasma using liquid chromatography-tandem mass spectrometry

Published:15 April 2018 DOI: 10.1016/j.jchromb.2018.03.014 PMID: 29550682
Claudia Spatari , Wenlong Li , Alfred H. Schinkel , Gaetano Ragno , Jan H.M. Schellens , Jos H. Beijnen , Rolf W. Sparidans

Abstract

A bio-analytical assay for the first third generation ALK inhibitor lorlatinib in mouse plasma was developed and validated. Ten-μl plasma samples were prepared by adding rucaparib as the internal standard and precipitation of the plasma proteins. For LC-MS/MS analysis, compounds were eluted at 0.5?mL/min and separated on a 3-μm particle-size, polar embedded octadecyl silica column by gradient elution using 0.1% of formic acid (in water) and methanol. Compounds were monitored with positive electrospray ionization using a triple quadrupole mass spectrometer in selected reaction monitoring mode. The assay was fully validated in the 2–2000?ng/mL calibration range. Within?day (8.0–11.6%) and between?day (10.0–15.0%) precisions and accuracies (99.0–113.3%) were within acceptable range. Plasma samples were deemed stable for 6?h at ambient temperature, during three freeze-thaw cycles and for 2?months at ?30?°C. Finally, the new assay was applied successfully to pilot pharmacokinetic studies in male and female wild-type mice.

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Materials
Procduct Name CAS Molecular Formula Supplier Price
Lorlatinib 1454846-35-5 C21H19FN6O2 342 suppliers $12.00-$2822.00
Lorlatinib 1454846-35-5 C21H19FN6O2 342 suppliers $12.00-$2822.00
Lorlatinib 1454846-35-5 C21H19FN6O2 342 suppliers $12.00-$2822.00
Lorlatinib 1454846-35-5 C21H19FN6O2 342 suppliers $12.00-$2822.00
PF0643922 1454846-35-5 - Inquiry
PF0643922 1454846-35-5 - Inquiry
PF0643922 1454846-35-5 - Inquiry
PF0643922 1454846-35-5 - Inquiry
PF0643922 1454846-35-5 - Inquiry
PF0643922 1454846-35-5 - Inquiry
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