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Analytical Chemistry

Analytical Chemistry

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Target-Triggered Assembly in a Nanopipette for Electrochemical Single-Cell Analysis

Published:10 December 2020 DOI: 10.1021/acs.analchem.0c04628 PMID: 33301293
Yi-Fan Ruan, Hai-Yan Wang, Xiao-Mei Shi, Yi-Tong Xu, Xiao-Dong Yu, Wei-Wei Zhao*, Hong-Yuan Chen, Jing-Juan Xu*

Abstract

Engineered nanopipette tools have recently emerged as a powerful approach for electrochemical nanosensing, which has major implications in both fundamental biological research and biomedical applications. Herein, we describe a generic method of target-triggered assembly of aptamers in a nanopipette for nanosensing, which is exemplified by sensitive and rapid electrochemical single-cell analysis of adenosine triphosphate (ATP), a ubiquitous energy source in life and important signaling molecules in many physiological processes. Specifically, a layer of thiolated aptamers is immobilized onto a Au-coated interior wall of a nanopipette tip. With backfilled pairing aptamers, the engineered nanopipette is then used for probing intracellular ATP via the ATP-dependent linkage of the split aptamers. Due to the higher surface charge density from the aptamer assembly, the nanosensor would exhibit an enhanced rectification signal. Besides, this ATP-responsive nanopipette tool possesses excellent selectivity and stability as well as high recyclability. This work provides a practical single-cell nanosensor capable of intracellular ATP analysis. More generally, integrated with other split recognition elements, the proposed mechanism could serve as a viable basis for addressing many other important biological species.

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Materials
Procduct Name CAS Molecular Formula Supplier Price
Adenosine 58-61-7 C10H13N5O4 1226 suppliers $6.00-$4581.40
Adenosine 58-61-7 C10H13N5O4 1226 suppliers $6.00-$4581.40
Adenosine 58-61-7 C10H13N5O4 1226 suppliers $6.00-$4581.40
Adenosine 58-61-7 C10H13N5O4 1226 suppliers $6.00-$4581.40
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Adenosine 58-61-7 C10H13N5O4 - Inquiry
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