一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Bioresource Technology >>article
Bioresource Technology

Bioresource Technology

IF: 9.7
Download PDF

Metabolic engineering of Escherichia coli for 1,3-propanediol biosynthesis from glycerol

Published:1 November 2018 DOI: 10.1016/j.biortech.2018.07.082 PMID: 30056370
Bo Yang , Shaoxiong Liang , Huanhuan Liu , Jiao Liu , Zhenzhen Cui , Jianping Wen

Abstract

In this study, the engineered E. coli was constructed for efficient transformation of glycerol to 1,3-propanediol (1,3-PDO). To regenerate NADPH, the key bottleneck in 1,3-PDO production, heterologous NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDN, encoded by gapN) pathway was introduced, and the gapN expression level was fine-tuned with specific 5′-untranslated regions (5′-UTR) to balance the carbon flux distribution between the metabolic pathways of NADPH regeneration and 1,3-PDO biosynthesis. Additionally, glucose was added to the medium to promote glycerol utilization and cell growth. To elevate the utilization of glycerol in the presence of glucose, E. coli JA11 was constructed through destroying PEP-dependent glucose transport system while strengthening the ATP-dependent transport system. Subsequent optimization of nitrogen sources further improved 1,3-PDO production. Finally, under the optimal fermentation condition, E. coli JA11 produced 13.47?g/L 1,3-PDO, with a yield of 0.64?mol/mol, increased by 325% and 100% compared with the original engineered E. coli JA03, respectively.

Substances (5)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Glycerol 56-81-5 C3H8O3 2251 suppliers $5.00-$6910.00
1,3-Propanediol 504-63-2 C3H8O2 699 suppliers $7.62-$1762.00
D-Glucose monohydrate 5996-10-1 C6H14O7 394 suppliers $29.00-$6717.78
NADPH 53-57-6 C21H30N7O17P3 80 suppliers $102.00-$21725.00
2-[PHOSPHONOOXY]-2-PROPENOIC ACID TRISODIUM SALT HYDRATE 5541-93-5 C3H4Na3O7P 6 suppliers Inquiry

Similar articles

IF:3.7

Biosynthesis of 1,3-Propanediol via a New Pathway from Glucose in Escherichia coli

ACS Synthetic Biology Mingda Li, Yang Zhang,etc Published: 14 June 2023
IF:4.3

Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose.

Microbial Cell Factories Qin Ke,?Chang Liu,etc Published: 3 September 2024
IF:3.7

Engineering Yarrowia lipolytica for the biosynthesis of geraniol

Metabolic Engineering Communications Ayushi Agrawal , Zhiliang Yang ,etc Published: 2 November 2023
芦溪县| 区。| 扎赉特旗| 通化县| 屏南县| 临高县| 望江县| 呼玛县| 沙洋县| 黄浦区| 南京市| 达拉特旗| 广饶县| 赤水市| 威宁| 通榆县| 喀喇沁旗| 和静县| 富顺县| 土默特左旗| 邵阳县| 卓资县| 高州市| 新化县| 沙坪坝区| 平湖市| 兴化市| 中方县| 防城港市| 枝江市| 巨鹿县| 望谟县| 金秀| 婺源县| 郸城县| 阜宁县| 永兴县| 乌兰浩特市| 封开县| 邳州市| 南汇区|