一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Metabolic Engineering Communications >>article
Metabolic Engineering Communications

Metabolic Engineering Communications

IF: 3.7
Download PDF

Engineering Yarrowia lipolytica for the biosynthesis of geraniol

Published:2 November 2023 DOI: 10.1016/j.mec.2023.e00228
Ayushi Agrawal , Zhiliang Yang , Mark Blenner

Abstract

Geraniol is a monoterpene with wide applications in the food, cosmetics, and pharmaceutical industries. Microbial production has largely used model organisms lacking favorable properties for monoterpene production. In this work, we produced geraniol in metabolically engineered Yarrowia lipolytica. First, two plant-derived geraniol synthases (GES) from Catharanthus roseus (Cr) and Valeriana officinalis (Vo) were tested based on previous reports of activity. Both wild type and truncated mutants of GES (without signal peptide targeting chloroplast) were examined by co-expressing with MVA pathway enzymes tHMG1 and IDI1. Truncated CrGES (tCrGES) produced the most geraniol and thus was used for further experimentation. The initial strain was obtained by overexpression of the truncated HMG1, IDI and tCrGES. The acetyl-CoA precursor pool was enhanced by overexpressing mevalonate pathway genes such as ERG10, HMGS or MVK, PMK. The final strain overexpressing 3 copies of tCrGES and single copies of ERG10, HMGS, tHMG1, IDI produced approximately 1?g/L in shake-flask fermentation. This is the first demonstration of geraniol production in Yarrowia lipolytica and the highest de novo titer reported to date in yeast.

Substances (8)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Geraniol 106-24-1 C10H18O 741 suppliers $6.00-$19950.00
Acetyl coenzyme A sodium salt 102029-73-2 C23H38N7O17P3S.3Na 291 suppliers $33.00-$2010.00
5-MALEIMIDO VALERIC ACID 57078-99-6 C9H11NO4 83 suppliers $26.00-$2920.00
Valerian, ext. 8057-49-6 43 suppliers Inquiry
Yarrowia lipolytica 10 suppliers Inquiry
IDI1 10 suppliers Inquiry
HMGS 1 suppliers Inquiry
AAMPS CATHARANTHUS ROSEUS MONOGRAPH 1 suppliers Inquiry

Similar articles

IF:9.7

Metabolic engineering of Escherichia coli for 1,3-propanediol biosynthesis from glycerol

Bioresource Technology Bo Yang , Shaoxiong Liang ,etc Published: 1 November 2018
IF:17.1

Phenylpropanoid biosynthesis.

Molecular Plant Thomas Vogt,etc Published: 1 January 2010
容城县| 高邑县| 洛宁县| 通榆县| 财经| 天全县| 平定县| 克什克腾旗| 吉木萨尔县| 锦屏县| 韩城市| 大庆市| 博客| 民权县| 喀什市| 凯里市| 合阳县| 嵊州市| 杨浦区| 大理市| 孙吴县| 察哈| 茌平县| 永仁县| 思茅市| 南宫市| 静乐县| 无为县| 田林县| 灵璧县| 长子县| 榆林市| 林芝县| 鹤庆县| 正阳县| 南陵县| 油尖旺区| 景洪市| 丘北县| 仙居县| 太白县|