一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Acta cirurgica brasileira >>article
Acta cirurgica brasileira

Acta cirurgica brasileira

IF: 1.1
Download PDF

The effect of hirudin on antagonisting thrombin induced apoptosis of human microvascular endothelial cells

Published:14 February 2019 DOI: 10.1590/s0102-865020190010000006 PMID: 30785507
Jiangying Zhu, Xinyuan Pan, Bojie Lin, Guanyu Lin, Rohan Pradhan, Feiwen Long, Guoqian Yin

Abstract

Purpose: To investigate whether hirudin exerts its antithrombin action to decrease the ratio of Human Microvascular Endothelial Cells (HMVECs) apoptosis.

Methods: Human microvascular endothelial cells (HMVECs) cultured in the third and fifth generations were used. HMVECs were divided into normal group, thrombin group (T group), natrual hirudin group (H group), thrombin + natrual hirudin group (T + H group), AG490 group, thrombin + AG490 group (T + AG490 group), natrual hirudin + AG490 group (H + AG490 group), thrombin + natural hirudin + AG490 (T + H + AG490 group).Apart from the normal group, the other groups were exposed to the relevant drugs for 24 hours.HMVEC apoptosis was assessed by flow cytometric and double Immunofluorescence of phosphorylation of JAK (P-JAK2) and TUNEL assay.

Results: Compared with the normal group, in thrombin group the HMVECs apoptosis rate were significantly increased (P<0.05).The results indicated that the index of apoptosis and the apoptosis rate were improved in cultures treated by natural hirudin (T + H group), relative to cultures with thrombin only (T group). We found that the index of apoptosis and the apoptosis rate in the AG490 + thrombin group were higher than that in the hirudin + thrombin group (P<0.05). Double Immunofluorescence of p-JAK2 and TUNEL assays showed that cells were double positive for P-JAK2 uptake and TUNEL detection liquid binding.

Conclusion: The natural hirudin and JAK2/STATs signal inhibitor AG490 could block the effects of thrombin. Natural hirudin could attenuate HMVECs apoptosis via antagonizing thrombin and it is suggested that this effect may occur by blocking the JAK2/STATs signaling pathway and this signaling pathways appears to be not the only pathway.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
AG 490 133550-30-8 C17H14N2O3 205 suppliers $12.00-$2885.00
AG 490 133550-30-8 C17H14N2O3 205 suppliers $12.00-$2885.00
AG 490 133550-30-8 C17H14N2O3 205 suppliers $12.00-$2885.00
AG 490 133550-30-8 C17H14N2O3 205 suppliers $12.00-$2885.00

Similar articles

IF:4.2

Protective effects of salidroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells

European journal of pharmacology Li Zhang , Huixin Yu ,etc Published: 14 June 2007
IF:19.8

Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery

Cell stem cell Zihang Pan, Qiyang Yao,etc Published: 22 November 2024
IF:4.8

The Benefits of Olive Oil for Skin Health: Study on the Effect of Hydroxytyrosol, Tyrosol, and Oleocanthal on Human Fibroblasts.

Nutrients Anabel González-Acedo, Javier Ramos-Torrecillas,etc Published: 25 April 2023
巴东县| 前郭尔| 锡林郭勒盟| 西乡县| 梧州市| 广昌县| 都安| 疏勒县| 三明市| 临沧市| 五河县| 芒康县| 金坛市| 巴东县| 罗江县| 亚东县| 唐山市| 闽清县| 武平县| 岳阳县| 高要市| 谷城县| 中江县| 景德镇市| 泰来县| 承德县| 无极县| 昔阳县| 千阳县| 中江县| 隆回县| 重庆市| 通江县| 新民市| 敦煌市| 勃利县| 永善县| 乌拉特后旗| 鲜城| 九江县| 通海县|