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Placenta

Placenta

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The effects of high-density lipoprotein and oxidized high-density lipoprotein on forskolin-induced syncytialization of BeWo cells

Published:1 January 2021 DOI: 10.1016/j.placenta.2020.10.024 PMID: 33160253
Hong-liang Wang , Ning Liang , Dong-xu Huang , Xiao-yan Zhao , Qin-yu Dang , Xin-yin Jiang , Rong Xiao , Huan-ling Yu

Abstract

Introduction

The negative relationship between maternal high-density lipoprotein-cholesterol (HDL-c) level during pregnancy and infant birth weight has been found. Syncytialization (differentiation and fusion) of trophoblast cells is important to fetal development. HDL has an antioxidant effect, and has been proved to protect trophoblast functions including hormone secretion and invasion. However, HDL is susceptible to oxidation, and high concentrations of HDL impair cell growth and oxidized HDL (oxHDL) inhibits cell proliferation and migration. Moreover, the effects of HDL and oxHDL on trophoblast syncytialization have not been characterized. The aim of this study was to investigate the effects of HDL and oxHDL on trophoblast syncytialization.

Methods

Human choriocarcinoma trophoblasts (BeWo cells) were treated with human HDL or oxHDL and then induced to differentiate by forskolin in syncytialization assays. Expression levels of mRNAs and proteins regulating syncytialization were detected by real-time PCR and western blotting, respectively.

Results

Treatments of HDL at high concentrations reduced human chorionic gonadotropin (hCG) secretion, placental alkaline phosphatase activity and fusion rates, and decreased the expressions of GCM1 and ERVW-1 mRNA as well as phospho-MAPK1/3 (p-MAPK1/3) and total MAPK1/3 protein in the forskolin-induced syncytialization of BeWo cells. Furthermore, treatment of oxHDL (20?μg/ml) decreased hCG secretion, but increased the expression of p-MAPK1/3 protein.

Discussion

These data suggested that both HDL at high concentrations and oxHDL inhibited BeWo cells syncytialization, and might be harmful to placental and fetal development.

Substances (8)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Forskolin 66575-29-9 C22H34O7 537 suppliers $12.00-$3200.00
Forskolin 66575-29-9 C22H34O7 537 suppliers $12.00-$3200.00
Forskolin 66575-29-9 C22H34O7 537 suppliers $12.00-$3200.00
Forskolin 66575-29-9 C22H34O7 537 suppliers $12.00-$3200.00
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Forskolin 66575-29-9 - Inquiry
Forskolin 66575-29-9 C22H34O7 - Inquiry
Forskolin 66575-29-9 C22H34O7 - Inquiry

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