一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Nucleic Acids Research >>article
Nucleic Acids Research

Nucleic Acids Research

IF: 16.6
Download PDF

CRISPR-iPAS: a novel dCAS13-based method for alternative polyadenylation interference

Published:21 March 2022 DOI: 10.1093/nar/gkac108 PMID: 35191504
Shuye Tian, Bin Zhang, Yuhao He, Zhiyuan Sun, Jun Li, Yisheng Li, Hongyang Yi, Yan Zhao, Xudong Zou, Yunfei Li, Huanhuan Cui, Liang Fang, Xin Gao, Yuhui Hu, Wei Chen

Abstract

Alternative polyadenylation (APA) plays an important role in gene regulation. With the recent application of novel sequencing technology in APA profiling, an ever-increasing number of APA genes/sites have been identified. However, the phenotypic relevance of most of these APA isoforms remains elusive, which is largely due to the lack of a convenient genetics tool for APA interference. To address this issue, herein, an efficient method is developed based on the CRISPR-dCas13 system, termed as CRISPR-iPAS. Out of eight different dCas13 proteins, Porphyromonas gulae (Pgu) dCas13b, is identified as the most effective one in blocking the usage of the polyadenylation site (PAS). With guide RNAs targeting at core regulatory elements, dPguCas13b enabled APA regulation of endogenous genes with different APA types, including tandem 3'UTR, alternative terminal exon, as well as intronic PAS. Finally, we demonstrated that the proposed APA perturbation tool could be used to investigate the functional relevance of APA isoforms.

Similar articles

IF:2.3

Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening

Biochemistry and Biophysics Reports Avani Mehta , Prateek Raj ,etc Published: 1 July 2021
IF:10.7

A novel pH-responsive hydrogel based on carboxymethyl cellulose/2-hydroxyethyl acrylate for transdermal delivery of naringenin

Carbohydrate Polymers So Hyun Park, Hyuk Soo Shin,etc Published: 15 November 2018
IF:2

Selamectin - a novel endectocide for dogs and cats.

Veterinary parasitology D E Jacobs,etc Published: 23 August 2000
广东省| 裕民县| 项城市| 陵川县| 新安县| 大厂| 阿拉善右旗| 怀宁县| 揭东县| 鄂伦春自治旗| 赤水市| 石林| 平定县| 营口市| 交城县| 寿光市| 宿州市| 永春县| 白城市| 高邮市| 巴林右旗| 牡丹江市| 乌兰浩特市| 文成县| 陵川县| 榆林市| 康定县| 福州市| 且末县| 西昌市| 罗源县| 乳源| 遵义市| 南京市| 延庆县| 神农架林区| 濮阳县| 舞钢市| 山丹县| 彭泽县| 宝鸡市|