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British Journal of Pharmacology

British Journal of Pharmacology

IF: 6.8
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DADLE promotes motor function recovery by inhibiting cytosolic phospholipase A2 mediated lysosomal membrane permeabilization after spinal cord injury.

Published:27 September 2023 DOI: 10.1111/bph.16255 PMID: 37766498
Yituo Chen, Haojie Zhang, Liting Jiang, Wanta Cai, Jiaxuan Kuang, Yibo Geng, Hui Xu, Yao Li, Liangliang Yang, Yuepiao Cai, Xiangyang Wang, Jian Xiao, Wenfei Ni, Kailiang Zhou

Abstract

Background and Purpose

Autophagy is a protective factor for controlling neuronal damage, while necroptosis promotes neuroinflammation after spinal cord injury (SCI). DADLE (D-Ala2, D-Leu5]-enkephalin) is a selective agonist for delta (δ) opioid receptor and has been identified as a promising drug for neuroprotection. The aim of this study was to investigate the mechanism/s by which DADLE causes locomotor recovery following SCI.

Experimental approach

Spinal cord contusion model was used and DADLE was given by i.p. (16?mg·kg?1) in mice for following experiments. Motor function was assessed by footprint and Basso mouse scale (BMS) score analysis. Western blotting used to evaluate related protein expression. Immunofluorescence showed the protein expression in each cell and its distribution. Network pharmacology analysis was used to find the related signalling pathways.

Key Results

DADLE promoted functional recovery after SCI. In SCI model of mice, DADLE significantly increased autophagic flux and inhibited necroptosis. Concurrently, DADLE restored autophagic flux by decreasing lysosomal membrane permeabilization (LMP). Additionally, chloroquine administration reversed the protective effect of DADLE to inhibit necroptosis. Further analysis showed that DADLE decreased phosphorylated cPLA2, overexpression of cPLA2 partially reversed DADLE inhibitory effect on LMP and necroptosis, as well as the promotion autophagy. Finally, AMPK/SIRT1/p38 pathway regulating cPLA2 is involved in the action DADLE on SCI and naltrindole inhibited DADLE action on δ receptor and on AMPK signalling pathway.

Conclusion and Implication

DADLE causes its neuroprotective effects on SCI by promoting autophagic flux and inhibiting necroptosis by decreasing LMP via activating δ receptor/AMPK/SIRT1/p38/cPLA2 pathway.

Substances (3)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
H-TYR-D-ALA-GLY-PHE-D-LEU-OH 63631-40-3 C29H39N5O7 106 suppliers $32.00-$2250.00
H-TYR-D-ALA-GLY-PHE-D-LEU-OH 63631-40-3 C29H39N5O7 106 suppliers $32.00-$2250.00

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