一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of Parasitology >>article
Journal of Parasitology

Journal of Parasitology

IF: 1.1
Download PDF

In Vitro and in Vivo Effect of MAPK Signal Transduction Pathway Inhibitors on Echinococcus multilocularis

Published:22 February 2019 DOI: 10.1645/18-121
Weifeng Gui,?Shuo Xu,?Z. Dang,?Yumin Zhao

Abstract

ABSTRACT To evaluate the effect of mitogen-activated protein kinase (MAPK) signal transduction pathway inhibitors against alveolar echinococcosis in vitro and in vivo, Echinococcus multilocularis metacestode cysts and protoscolices were obtained from infected mice. Protein chip technology was utilized to screen for key highly expressed target proteins in the MAPK pathway in this parasite and their corresponding inhibitors. Four-week-old Balb/c female mice used for the in vivo experiment underwent inoculation of E. multilocularis by intraperitoneal injection, as well as intragastric administration of MAPK inhibitors for 6 wk. We included 6 groups of mice: a phosphate-buffered saline (PBS) group (negative control); an albendazole-treated group (positive group); and 4 experimental groups treated with TRx0237 mesylate, GDC-0994, pifithrin-β hydrobromide, or Selonsertib. Echinococcus multilocularis protoscolices were collected and cultured in 1066 medium with penicillin/streptomycin and 10% fetal bovine serum. The in vitro experiment included a PBS group (negative control), a dimethyl sulfoxide–treated group (solvent group), and 4 inhibitor-treated groups as in the in vivo experiment (experimental groups). Each inhibitor group received 4 drug concentrations (5, 30, 55, and 80 μM), and the experiment was performed in triplicate per sample. Fluorescence microscopy was used to evaluate the survival rate of the protoscolices every 48 hr beginning from the first 24 hr. The same grouping was used to evaluate cytotoxicity on E. multilocularis germinal cells and L02 cells. The average weights of E. multilocularis metacestode cyst tissue from each group of the in vivo experiment were 873 mg (PBS), 335 mg (albendazole), 323 mg (TRx0237 mesylate), 420 mg (GDC-0994), 340 mg (pifithrin-β hydrobromide), and 642 mg (Selonsertib). Results showed albendazole, TRx0237 mesylate, and pifithrin-β hydrobromide had significant inhibitory effects on inhibition of E. multilocularis. We found a positive correlation between drug concentrations and the inhibitory effects seen in the in vitro experiment, with the differences in contrast with the control group becoming statistically significant after 72 hr of treatment (P < 0.05). The inhibition rates of TRx0237 mesylate to germinal cells by drug concentration were 23.73, 46.59, 74.71, and 77.44%. Other drugs had no effect on germinal cells. All the inhibitors had low toxicity on L02 cells. Inhibitors of the MAPK signal transduction pathway showed significant inhibitory effects on E. multilocularis, suggesting these may be potential candidates for the treatment of alveolar echinococcosis.

Substances (6)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
TRX-0237 mesylate 1236208-20-0 C16H19N3S.2(CH4O3S) 87 suppliers Inquiry
TRX-0237 mesylate 1236208-20-0 C16H19N3S.2(CH4O3S) 87 suppliers Inquiry
TRX-0237 mesylate 1236208-20-0 C16H19N3S.2(CH4O3S) 87 suppliers Inquiry
TRX-0237 mesylate 1236208-20-0 C16H19N3S.2(CH4O3S) 87 suppliers Inquiry

Similar articles

IF:5.7

4-Methylumbelliferone Inhibits Angiogenesis in Vitro and in Vivo

Journal of Agricultural and Food Chemistry Javier A. García-Vilas, Ana R. Quesada,etc Published: 12 April 2013
IF:5.4

Protective effects and mechanism of puerarin targeting PI3K/Akt signal pathway on neurological diseases.

ACS Applied Energy Materials Qian Wang, Zi-Nuo Shen,etc Published: 24 October 2022
IF:4.6

In Vitro and In Vivo Neuroprotective Effects of Sarcosine.

ACS Applied Bio Materials Arzugül Tanas, ?zlem ?zdemir Tozlu,etc Published: 15 October 2022
克山县| 灵石县| 五指山市| 商城县| 扬州市| 东至县| 菏泽市| 新蔡县| 砀山县| 峨边| 长顺县| 天镇县| 浮梁县| 庆安县| 濉溪县| 东阳市| 和平区| 耿马| 盐源县| 白银市| 隆昌县| 黄石市| 通州市| 敖汉旗| 大荔县| 东山县| 武穴市| 台山市| 封丘县| 奉化市| 彭州市| 南雄市| 定远县| 临城县| 玉林市| 乌审旗| 太和县| 高州市| 奎屯市| 平湖市| 禄丰县|