一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Pest Management Science >>article
Pest Management Science

Pest Management Science

IF: 3.8
Download PDF

The determination of Plutella xylostella (L.) GSTs (PxGSTs) involved in the detoxification metabolism of Tolfenpyrad

Published:9 June 2020 DOI: 10.1002/ps.5958 PMID: 32515133
Yifan Li, Hong Sun, Zhen Tian, Xinxin Su, Yue Li, Xuan Ye, Yifei Zhou, Shengli Zheng, Jiyuan Liu, Yalin Zhang

Abstract

BACKGROUND

Insect glutathione S-transferases (GSTs) play a crucial role in insecticide detoxification. However, there remains a distinct lack of information regarding the role of GSTs in the detoxification of Tolfenpyrad (TFP) in insects.

RESULTS

Real-time quantitative PCR showed significant upregulation of PxGSTs after exposure to TFP for 6?h. An in vitro inhibition assay showed that TFP could inhibit PxGSTδ, PxGSTε and PxGSTσ, and the most pronounced inhibitory effect was on PxGSTσ. Metabolism assays displayed that PxGSTσ was superior to other test PxGSTs in metabolizing TFP. The molecular docking of TFP and PxGSTσ revealed that the H-bond provided by the sidechains of Tyr107 and Tyr162 were key to the detoxification of TFP by PxGSTσ. Further tests using mutant PxGSTσ proteins at the sites of Tyr107 (PxGSTσY107A) and Tyr162 (PxGSTσY162A) corroborated that the individual replacement of Tyr107 and Tyr162 could greatly weaken the binding and metabolic abilities to TFP.

CONCLUSION

Metabolic interactions between the Plutella xylostella (L.) GSTs (PxGSTs) and TFP were deciphered. This study illustrates the molecular metabolism mechanism of PxGSTσ towards TFP and provides theoretical underpinnings for the design and optimization of novel TFP-like insecticides. ? 2020 Society of Chemical Industry

Substances (33)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
Imidacloprid 138261-41-3 C9H10ClN5O2 575 suppliers $33.00-$1683.00
Imidacloprid 138261-41-3 C9H10ClN5O2 575 suppliers $33.00-$1683.00
Imidacloprid 138261-41-3 C9H10ClN5O2 575 suppliers $33.00-$1683.00
Imidacloprid 138261-41-3 C9H10ClN5O2 575 suppliers $33.00-$1683.00
2-Pyrazinecarboxylic acid 98-97-5 C5H4N2O2 519 suppliers $4.00-$2743.13
2-Pyrazinecarboxylic acid 98-97-5 C5H4N2O2 519 suppliers $4.00-$2743.13
2-Pyrazinecarboxylic acid 98-97-5 C5H4N2O2 519 suppliers $4.00-$2743.13
2-Pyrazinecarboxylic acid 98-97-5 C5H4N2O2 519 suppliers $4.00-$2743.13
Rotenone 83-79-4 C23H22O6 457 suppliers $20.00-$1460.00
Rotenone 83-79-4 C23H22O6 457 suppliers $20.00-$1460.00

Similar articles

IF:3.4

Electrochemical Sensor for the Determination of Methylthiouracil in Meat Samples.

Sensors Andrea Marco, Antonio Canals,etc Published: 15 November 2022
IF:3.2

Role of MOTS-c in the regulation of bone metabolism.

Frontiers in Physiology Xuejie Yi, Guangxuan Hu,etc Published: 1 January 2023
IF:2.3

Efficacy of the Dietary Supplement S-Adenosyl-L-Methionine

Annals of Pharmacotherapy CW Fetrow,?J. R. Avila,etc Published: 1 November 2001
肥西县| 宣化县| 遵义县| 平和县| 富宁县| 皋兰县| 铁力市| 芜湖县| 新昌县| 平山县| 木里| 黑龙江省| 邻水| 洪江市| 金昌市| 珠海市| 安图县| 隆林| 木兰县| 温泉县| 贡觉县| 茶陵县| 沅江市| 蓝山县| 沈阳市| 泸定县| 尚志市| 梓潼县| 化隆| 麻栗坡县| 太湖县| 自治县| 石楼县| 惠安县| 三亚市| 汉中市| 临猗县| 漳浦县| 浦北县| 新安县| 昌图县|