一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Jove-Journal of Visualized Experiments >>article
Jove-Journal of Visualized Experiments

Jove-Journal of Visualized Experiments

IF: 1.2
Download PDF

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering.

Published:26 October 2016 DOI: 10.3791/53704
T. Christiani,?K. Toomer,?J. Sheehan,?A. Nitzl,?A. Branda,?E. England,?P. Graney,?C. Iftode,?A. Vernengo

Abstract

Injectable biomaterials are defined as implantable materials that can be introduced into the body as a liquid and solidify in situ. Such materials offer the clinical advantages of being implanted minimally invasively and easily forming space-filling solids in irregularly shaped defects. Injectable biomaterials have been widely investigated as scaffolds for tissue engineering. However, for the repair of certain load-bearing areas in the body, such as the intervertebral disc, scaffolds should possess adhesive properties. This will minimize the risk of dislocation during motion and ensure intimate contact with the surrounding tissue, providing adequate transmission of forces. Here, we describe the preparation and characterization of a scaffold composed of thermally sensitive poly(N-isopropylacrylamide)-graft-chondroitin sulfate (PNIPAAM-g-CS) and alginate microparticles. The PNIPAAm-g-CS copolymer forms a viscous solution in water at RT, into which alginate particles are suspended to enhance adhesion. Above the lower critical solution temperature (LCST), around 30 °C, the copolymer forms a solid gel around the microparticles. We have adapted standard biomaterials characterization procedures to take into account the reversible phase transition of PNIPAAm-g-CS. Results indicate that the incorporation of 50 or 75 mg/ml?alginate particles into 5% (w/v) PNIPAAm-g-CS solutions quadruple the adhesive tensile strength of PNIPAAm-gCS alone (p<0.05). The incorporation of alginate microparticles also significantly increases swelling capacity of PNIPAAm-g-CS (p<0.05), helping to maintain a space-filling gel within tissue defects. Finally, results of the in vitro toxicology assay kit, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and Live/Dead viability assay indicate that the adhesive is capable of supporting the survival and proliferation of encapsulated Human Embryonic Kidney (HEK) 293 cells over 5 days.

Substances (6)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Sodium alginate 9005-38-3 C5H7O4COONa 794 suppliers $12.70-$3270.00
XTT sodium salt 111072-31-2 C22H18N7NaO13S2 228 suppliers $45.00-$1190.00
POLY(N-ISOPROPYL ACRYLAMIDE) 25189-55-3 C6H11NO 96 suppliers $29.00-$902.00
Human embryonic kidney cells 293 C18 9 suppliers Inquiry
Alginate Antibody 8 suppliers Inquiry
XTT (2,3-BIS-(2-METHOXY-4 5 suppliers Inquiry

Similar articles

IF:5.8

Highly stretchable self-healing poly(N,N-dimethylacrylamide) hydrogels

European Polymer Journal Melek Pamuk Algi , Oguz Okay,etc Published: 1 October 2014
赣州市| 拜泉县| 浠水县| 易门县| 聂拉木县| 汉沽区| 延寿县| 买车| 博白县| 隆回县| 阜宁县| 历史| 蒙阴县| 香河县| 达孜县| 沅江市| 黄陵县| 敖汉旗| 盐城市| 柘城县| 定边县| 当阳市| 昆明市| 宜兰市| 和顺县| 肥乡县| 松溪县| 砀山县| 黑山县| 高邑县| 绥江县| 灌阳县| 新化县| 浮梁县| 闸北区| 任丘市| 钦州市| 阳泉市| 大冶市| 英超| 郁南县|