一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> PLoS ONE >>article
PLoS ONE

PLoS ONE

IF: 2.9
Download PDF

Tannic acid, an IL-1β-direct binding compound, ameliorates IL-1β-induced inflammation and cartilage degradation by hindering IL-1β-IL-1R1 interaction

Published:1 January 2023 DOI: 10.1371/journal.pone.0281834 PMID: 37079558
Hae-Ri Lee, Young-Jin Jeong, Joong-Woon Lee, JooYeon Jhun, Hyun Sik Na, Keun-Hyung Cho, Seok Jung Kim, Mi-La Cho, Tae-Hwe Heo

Abstract

Interleukin-1β (IL-1β) is one of the most potent pro-inflammatory cytokines implicated in a wide range of autoinflammatory, autoimmune, infectious, and degenerative diseases. Therefore, many researchers have focused on developing therapeutic molecules that inhibit IL-1β-IL-1 receptor 1 (IL-1R1) interaction for the treatment of IL-1-related diseases. Among IL-1-related diseases, osteoarthritis (OA), is characterized by progressive cartilage destruction, chondrocyte inflammation, and extracellular matrix (ECM) degradation. Tannic acid (TA) has been proposed to have multiple beneficial effects, including anti-inflammatory, anti-oxidant, and anti-tumor activities. However, it is unclear whether TA plays a role in anti-IL-1β activity by blocking IL-1β-IL-1R1 interaction in OA. In this study, we report the anti-IL-1β activity of TA in the progression of OA in both in vitro human OA chondrocytes and in vivo rat OA models. Herein, using-ELISA-based screening, natural compound candidates capable of inhibiting the IL-1β-IL-1R1 interaction were identified. Among selected candidates, TA showed hindering IL-1β-IL-1R1 interaction by direct binding to IL-1β using surface plasmon resonance (SPR) assay. In addition, TA inhibited IL-1β bioactivity in HEK-Blue IL-1-dependent reporter cell line. TA also inhibited IL-1β-induced expression of inducible nitric oxide synthase (NOS2), cyclooxygenase-2 (COX-2), IL-6, tumor necrosis factor-alpha (TNF-α), nitric oxide (NO), and prostaglandin E2 (PGE2) in human OA chondrocytes. Moreover, TA downregulated IL-1β-stimulated matrix metalloproteinase (MMP)3, MMP13, ADAM metallopeptidase with thrombospondin type 1 motif (ADAMTS)4, and ADAMTS5, while upregulating collagen type II (COL2A1) and aggrecan (ACAN). Mechanistically, we confirmed that TA suppressed IL-1β-induced MAPK and NF-κB activation. The protective effects of TA were also observed in a monosodium iodoacetamide (MIA)-induced rat OA model by reducing pain and cartilage degradation and inhibiting IL-1β-mediated inflammation. Collectively, our results provide evidence that TA plays a potential role in OA and IL-1β-related diseases by hindering IL-1β-IL-1R1 interaction and suppressing IL-1β bioactivity.

Similar articles

IF:7.1

IL-17A is a key regulator of neuroinflammation and neurodevelopment in cognitive impairment induced by sevoflurane

Free Radical Biology and Medicine Qi Zhang, Yanan Li,etc Published: 22 November 2024
IF:3.3

Curcumin ameliorates astrocyte inflammation through AXL in cuprizone-induced mice

Toxicology and applied pharmacology Wenjing Zhang, Mengjiao Sun,etc Published: 23 November 2024
海阳市| 宁明县| 石家庄市| 遂昌县| 海丰县| 望江县| 纳雍县| 金寨县| 宁强县| 蛟河市| 开远市| 石林| 高邑县| 花莲县| 米易县| 洪泽县| 都匀市| 长垣县| 玉田县| 勐海县| 侯马市| 云龙县| 陈巴尔虎旗| 石首市| 海兴县| 曲水县| 巴里| 邯郸市| 报价| 诸城市| 巴林右旗| 吴旗县| 大关县| 萝北县| 同德县| 河南省| 吴堡县| 昌都县| 通化市| 乌拉特中旗| 岚皋县|