2-Aminoadenosine synthesis
- Product Name:2-Aminoadenosine
- CAS Number:2096-10-8
- Molecular formula:C10H14N6O4
- Molecular Weight:282.26
Yield:2096-10-8 97%
Reaction Conditions:
with purine nucleoside phosphorylase;uridine phosphorylase in aq. phosphate buffer;dimethyl sulfoxide at 60; pH=7; for 5 h;Enzymatic reaction;Solvent;Temperature;
Steps:
12.1
Assays were performed in 5 and 10% of aprotic dipolar co-solvents, at 60°C, twelve units/ml cell lysate wereadded to 150 ml of a solution kept thermostatically at 60 °C, and having the following composition of substrate solutions:1. 15 mM Uridine/2’-Deoxyuridine,2. 5 mM 2,6-Diaminopurine, and3. 30 mM potassium phosphate buffer, pH 7.[0084] After 5 hours, the reaction mixture was filtered by centrifugation at 2000 3g for 30 min, at 4°C, through anAmicon ultra-4 Centrifugal Filter Devices (Millipore, Bedford, MA) with a 3000-Da cut-off, and the filtrate was recovered.In table 3, the production yields of 2,6-Diaminopurine nucleosides prepared in the presence of co-solvents is shown.The resulting 2,6-Diaminopurine nucleosides (2,6-Diaminopurine riboside or 2,6-Diaminopurine deoxyriboside) wereanalyzed by HPLC. In these reactions, the products were obtained in high yields (over 80%).
References:
EP2516637,2016,B1 Location in patent:Paragraph 0083-0084
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