Fmoc-4-nitro-L-phenylalanine synthesis
- Product Name:Fmoc-4-nitro-L-phenylalanine
- CAS Number:95753-55-2
- Molecular formula:C24H20N2O6
- Molecular Weight:432.43
949-99-5
82911-69-1
95753-55-2
GENERAL STEPS: 37.7 g (0.179mol) of (S)-2-amino-3-(4-nitrophenyl)propionic acid was suspended in 200 mL of a 2% NaOH aqueous solution, and 80 mL of acetonitrile was added until completely dissolved. To this solution was added 61.8 g (0.183 mol) of 9-fluorenylmethyl-N-succinimidyl carbonate suspended in 100 mL of acetonitrile. The reaction mixture was stirred at room temperature for 3 h and subsequently acidified to pH 4-5 with 5% HCl. The precipitated white crystals were collected by filtration, washed with ethyl acetate and dried. The product was recrystallized by ethanol-dioxane (50:50) solvent mixture. The yields were 67% (3a) and 71% (3b), respectively. The melting point was 230 °C (literature value 233-234 °C [10]).IR spectrum (ν, cm-1): 3429, 3202, 1724, 1693, 1601, 1520, 1447, 1354, 1223, 1057, 856, 760, 741.1H NMR spectrum (DMSO-d6, δ, ppm): 2.90- 3.09 (m, 1H, CH2), 3.25 (dd, 1H, CH2, 2J=13.7, 3J=4.3 Hz), 4.13-4.34 (m, 4H, 2CH2, 2CH), 7.23-7.45 (m, 4Harom), 7.54 (d, 2Harom, 3J=8.6 Hz), 7.60-7.64 (m, 2Harom), 7.79 (d, 1H, NH, 3J=8.6 Hz), 7.88 (d, 2Harom, 3J=7.5 Hz), 8.14 (d, 2Harom, 3J=8.6 Hz), 12.93 (s, 1H, COOH).13C NMR spectra (DMSO-d6, δ, ppm): 36.62 (CH2) 47.03 (CH), 55.25 (CH), 66.03 (CH2), 120.56, 123.69, 125.62, 127.45, 128.07, 130.94, 141.16, 144.20, 146.73 (arom), 156.39 (CONH), 173.27 (COOH). Mass spectrum (ESI, m/z): 455.1214 [M+Na]+. Molecular formula C24H20N2O6, calculated molecular weight 432.1321.
63-91-2
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95753-55-2
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Yield:-
Steps:
Multi-step reaction with 2 steps
1: nitric acid; sulfuric acid / 3 h / 8 - 10 °C
2: sodium hydroxide / water; acetonitrile / 3 h / 20 °C
References:
Tolstyakov;Tolstobrova;Zarubina;Popova;Protas;Chuprun;Trifonov [Russian Journal of Organic Chemistry,2016,vol. 52,# 11,p. 1681 - 1685][Zh. Org. Khim.,2016,vol. 52,# 11,p. 1686 - 1690,5]
949-99-5
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82911-69-1
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95753-55-2
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28920-43-6
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949-99-5
366 suppliers
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95753-55-2
242 suppliers
$7.00/250mg