L-tert-Leucine synthesis
- Product Name:L-tert-Leucine
- CAS Number:20859-02-3
- Molecular formula:C6H13NO2
- Molecular Weight:131.17
815-17-8
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20859-02-3
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Yield:20859-02-3 80%
Reaction Conditions:
with ammonium hydroxide;D-glucose;ammonium chloride;sodium hydroxide in water; pH=8.3; for 5.5 h;Enzymatic reaction;stereoselective reaction;Time;Reagent/catalyst;
Steps:
2 2.8 Reductive amination of TMP to l-tert-leucine in liter-scale
Bioconversion of TMP to l-tert-leucine was carried out by recombinant whole-cells harboring pET28a-E-G. In a reactor (2L), 1L of water was added to a mixture of 178.2g glucose (0.9mol), 78.1g TMP (0.6mol), 26.8g NH4Cl (0.5mol) and 10.0g of the whole cells of E. coli BL21/pET28a-E-G. The reaction pH was adjusted to 8.5 with NaOH solution. During the reaction process, the pH was automatically adjusted to 8.5 by titrating 50% (v/v) NH3·H2O. When the reaction was terminated, the reaction mixture was heated to 60°C, followed by centrifugation to remove the biocatalysts. The remained solution was adjusted to pH 5.9 and concentrated via rotary evaporator. Then the solution temperature was decreased slowly to 25°C and continued to decrease to 4°C, the l-tert-leucine crystals were obtained.
References:
Li, Jing;Pan, Jiang;Zhang, Jie;Xu, Jian-He [Journal of Molecular Catalysis B: Enzymatic,2014,vol. 105,p. 11 - 17]
62965-35-9
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20859-02-3
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