Glycine synthesis
- Product Name:Glycine
- CAS Number:56-40-6
- Molecular formula:C2H5NO2
- Molecular Weight:75.07
543-24-8
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$9.00/100g
56-40-6
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$5.00/25g
Yield:56-40-6 100 %Spectr.
Reaction Conditions:
Stage #1: N-acetylglycinewith sodium hydroxideHeating;Sonication;
Stage #2: with porcine kidney N-acetylase;water in aq. phosphate buffer at 37; for 0.00833333 h;Enzymatic reaction;
Steps:
6.e Example 6e DNP Hyperpolarization of N-Acetyl Glycine and Dissolution Following which the Presence of N-Acetylase for Hydrolysis
35 mg of N-acetyl glycine (117.1 g/mol, 0.298 mmol) was dissolved in 26 μl 12 M NaOH. Heating, sonication and vortexing was applied to create a clear glassing solution. The total weight of the sample was 71 mg (50 μl weigh 65 mg). 1.05 mg Radical 1 and 1.5 μl of a 3-Gd stock solution (14.5 mmol/g) was added. The total N-acetyl glycine concentration in the sample was 5.5 M, the radical concentration was 13.5 mM, and the 3-Gd concentration was 0.4 mM. (0286) 35.8 mg of the composition was transferred from the Eppendorf tube to a sample cup and the sample cup was inserted into a DNP polariser. The composition was hyperpolarized under DNP conditions for 60 min at 1.2 K in a 3.35 T magnetic field under irradiation with microwave (93.900 GHz). (0287) The sample was dissolved in 7 ml 40 mM phosphate buffer (40 mM, pH 7.3). 1 ml of the hyperpolarized N-acetyl glycine solution was injected through a transfer line into a 10 mm NMR tube placed at 14.1 T and 37° C. The 10 mm NMR tube contained a 500 μl phosphate solution of approximately 230 U of an acylase (5.2 mg) from porcine kidney. A time series of 5 degrees 1D 13C-NMR spectra was recorded with a total delay between the pulses of 3 s. (0288) The N-acetyl glycine was fully hydrolyzed after 30 s.
References:
US9694090,2017,B2 Location in patent:Page/Page column 33; 34
1668-10-6
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56-40-6
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79-11-8
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56-40-6
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4530-20-5
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56-40-6
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1738-68-7
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56-40-6
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