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ChemicalBook CAS DataBase List L-Tetrahydrofolic Acid
135-16-0

L-Tetrahydrofolic Acid synthesis

2synthesis methods
Tetrahydrofolate is a reduced derivative of folic acid, a coenzyme obtained by the reduction of dihydrofolate by dihydrofolate reductase.
-

Yield:-

Steps:

Multi-step reaction with 2 steps
1: 79 percent / ascorbic acid, sodium hydroxide, sodium dithionite / H2O / 0.08 h / Ambient temperature
2: NADPH, bovine liver dihydrofolate reductase, phosphate buffer / 1.67 h / 30 °C

References:

Charlton, Peter A.;Young, Douglas W.;Birdsall, Berry;Feeney, James;Roberts, Gordon C. K. [Journal of the Chemical Society. Perkin transactions I,1985,p. 1349 - 1354]

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